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Literature summary extracted from
- Properties and kinetics of glucan phosphorylase of the amylose-forming yeast Cryptococcus laurentii (1978), Z. Allg. Mikrobiol., 18, 95-106.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.1 | Cl- | weak inhibition | Papiliotrema laurentii |  |
| 2.4.1.1 | NaNO3 | strong inhibition | Papiliotrema laurentii | |
| 2.4.1.1 | sulfate | weak | Papiliotrema laurentii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.1 | additional information | - |
additional information | - |
Papiliotrema laurentii | |
| 2.4.1.1 | 16 | - |
glucose 1-phosphate | - |
Papiliotrema laurentii | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.1 | Papiliotrema laurentii | - |
syn. Rhodotorula peneaus, strain 48-23A | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.1 | partial purification | Papiliotrema laurentii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.1 | 1.76 | - |
- |
Papiliotrema laurentii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.1 | glycogen + glucose 1-phosphate | - |
Papiliotrema laurentii | glycogen + phosphate | - |
r | |
| 2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Papiliotrema laurentii | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.1 | 36 | - |
20% loss of activity within 5 min | Papiliotrema laurentii |
| 2.4.1.1 | 43 | - |
and above, inactivation within 5 min | Papiliotrema laurentii |
| 2.4.1.1 | 45 | - |
complete loss of activity after 5 min | Papiliotrema laurentii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.1 | 5.9 | 6.2 | - |
Papiliotrema laurentii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.1 | 5.2 | 7.5 | approx. 70% of maximal activity at pH 5.2 and approx. half-maximal activity at pH 7.5 | Papiliotrema laurentii |
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Release 2023.1 (January 2023)
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