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Literature summary extracted from

  • Shashi, K.; Bachhawat, A.K.; Joseph, R.
    ATP:citrate lyase of Rhodotorula gracilis: purification and properties (1990), Biochim. Biophys. Acta, 1033, 23-30.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.8 NH4+ stimulates Rhodotorula toruloides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.8 ADP 1 mM Rhodotorula toruloides
2.3.3.8 AMP 1 mM Rhodotorula toruloides
2.3.3.8 D-glucose 6-phosphate
-
Rhodotorula toruloides
2.3.3.8 Glu
-
Rhodotorula toruloides
2.3.3.8 lauroyl-CoA
-
Rhodotorula toruloides
2.3.3.8 myristoyl-CoA
-
Rhodotorula toruloides
2.3.3.8 oleoyl-CoA
-
Rhodotorula toruloides
2.3.3.8 palmitoyl-CoA
-
Rhodotorula toruloides
2.3.3.8 stearoyl-CoA
-
Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.8 0.175
-
ATP
-
Rhodotorula toruloides
2.3.3.8 1.25
-
citrate 0.2 M Tris-HCl buffer containing 0.25 M KCl Rhodotorula toruloides
2.3.3.8 2.63
-
citrate in 0.1 M Tris-HCl buffer Rhodotorula toruloides
2.3.3.8 3.44
-
citrate in Tris-acetate buffer Rhodotorula toruloides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.8 120000
-
4 * 120000, the two minor proteins 51000 Da and 49000 Da can be the result of proteolytic cleavage of ATP:citrate lyase by an endogenous trypsin-like proteinase Rhodotorula toruloides
2.3.3.8 520000
-
gel filtration Rhodotorula toruloides

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.8 Rhodotorula toruloides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.8
-
Rhodotorula toruloides

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
2.3.3.8 -20°C, 20% glycerol, stable up to 2 months Rhodotorula toruloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.8 ATP + citrate + CoA
-
Rhodotorula toruloides ADP + phosphate + acetyl-CoA + oxaloacetate
-
?

Subunits

EC Number Subunits Comment Organism
2.3.3.8 tetramer 4 * 120000, the two minor proteins 51000 Da and 49000 Da can be the result of proteolytic cleavage of ATP:citrate lyase by an endogenous trypsin-like proteinase Rhodotorula toruloides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.8 37
-
-
Rhodotorula toruloides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.8 8.4
-
-
Rhodotorula toruloides