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Literature summary extracted from

  • Sutherlin, A.; Hedl, M.; Sanchez-Neri, B.; Burgner, J.W.; Stauffacher, C.V.; Rodwell, V.W.
    Enterococcus faecalis 3-hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis (2002), J. Bacteriol., 184, 4065-4070.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.10 MgCl2
-
 Gallus gallus
2.3.3.10 MgCl2 optimal activity in 2.0 mM Enterococcus faecalis

Application

EC Number Application Comment Organism
2.3.3.10 medicine
-
 Homo sapiens
2.3.3.10 medicine potential of the mevalonate pathway enzymes of enterococci as targets for antibiotics Enterococcus faecalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.10
-
 Gallus gallus
2.3.3.10 cloning of a gene cluster encoding enzymes responsible for the mevalonate pathway Streptomyces sp.
2.3.3.10 expression of recombinant wild-type cytoplasmic and Cys129 mutant enzymes Homo sapiens
2.3.3.10 mvaS gene isolated, expressed in Escherichia coli from a pET28 vector Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.0012
-
 acetoacetyl-CoA
-
 Gallus gallus
2.3.3.10 0.0015
-
 acetyl-CoA
-
 Blattella germanica
2.3.3.10 0.01
-
 acetoacetyl-CoA
-
 Enterococcus faecalis
2.3.3.10 0.27
-
 acetyl-CoA
-
 Gallus gallus
2.3.3.10 0.35
-
 acetyl-CoA
-
 Enterococcus faecalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 cytoplasm
-
 Gallus gallus 5737
-
2.3.3.10 cytoplasm
-
 Homo sapiens 5737
-
2.3.3.10 cytoplasm
-
 Rattus norvegicus 5737
-
2.3.3.10 cytoplasm
-
 Bos taurus 5737
-
2.3.3.10 cytosol
-
 Gallus gallus 5829
-
2.3.3.10 cytosol
-
 Homo sapiens 5829
-
2.3.3.10 cytosol
-
 Rattus norvegicus 5829
-
2.3.3.10 cytosol
-
 Mesocricetus auratus 5829
-
2.3.3.10 cytosol
-
 Blattella germanica 5829
-
2.3.3.10 mitochondrion
-
 Gallus gallus 5739
-
2.3.3.10 mitochondrion
-
 Mus musculus 5739
-
2.3.3.10 mitochondrion
-
 Homo sapiens 5739
-
2.3.3.10 mitochondrion
-
 Rattus norvegicus 5739
-
2.3.3.10 mitochondrion
-
 Sus scrofa 5739
-
2.3.3.10 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 42000
-
 2 * 42000, calculated from amino acid sequence Enterococcus faecalis
2.3.3.10 45000
-
 2 * 45000, SDS-PAGE Enterococcus faecalis
2.3.3.10 83900
-
 analytical ultracentrifugation Enterococcus faecalis
2.3.3.10 84300
-
 calculated from amino acid sequence Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Staphylococcus aureus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Mus musculus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Homo sapiens
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Sus scrofa
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Bos taurus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Streptococcus pneumoniae
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Streptococcus pyogenes
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Mesocricetus auratus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Staphylococcus epidermidis
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Staphylococcus haemolyticus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Streptomyces sp.
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Borreliella burgdorferi
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Blattella germanica
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Enterococcus faecalis isopentenyl diphosphate biosynthesis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Blattella germanica
-
 cockroach
-
2.3.3.10 Borreliella burgdorferi
-
-
-
2.3.3.10 Bos taurus
-
 ox
-
2.3.3.10 Enterococcus faecalis
-
-
-
2.3.3.10 Gallus gallus
-
 avian
-
2.3.3.10 Homo sapiens
-
 human
-
2.3.3.10 Mesocricetus auratus
-
 syrian hamster, golden hamster
-
2.3.3.10 Mus musculus
-
 mouse
-
2.3.3.10 Rattus norvegicus
-
 rat
-
2.3.3.10 Staphylococcus aureus
-
-
-
2.3.3.10 Staphylococcus epidermidis
-
-
-
2.3.3.10 Staphylococcus haemolyticus
-
-
-
2.3.3.10 Streptococcus pneumoniae
-
-
-
2.3.3.10 Streptococcus pyogenes
-
-
-
2.3.3.10 Streptomyces sp.
-
-
-
2.3.3.10 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10
-
 Homo sapiens
2.3.3.10 recombinant enzyme Enterococcus faecalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.10 liver
-
 Gallus gallus
-
2.3.3.10 liver
-
 Mus musculus
-
2.3.3.10 liver
-
 Rattus norvegicus
-
2.3.3.10 liver
-
 Sus scrofa
-
2.3.3.10 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 10
-
-
 Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Staphylococcus aureus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Mus musculus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Sus scrofa (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Sus scrofa (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Bos taurus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Enterococcus faecalis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Streptococcus pneumoniae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Streptococcus pyogenes (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Mesocricetus auratus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Staphylococcus epidermidis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Staphylococcus haemolyticus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Streptomyces sp. (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Borreliella burgdorferi (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Blattella germanica (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O isopentenyl diphosphate biosynthesis Enterococcus faecalis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Subunits

EC Number Subunits Comment Organism
2.3.3.10 dimer
-
 Gallus gallus
2.3.3.10 dimer
-
 Homo sapiens
2.3.3.10 dimer
-
 Bos taurus
2.3.3.10 dimer 2 * 45000, SDS-PAGE Enterococcus faecalis
2.3.3.10 dimer 2 * 42000, calculated from amino acid sequence Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.10 37
-
-
 Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.10 9.8
-
-
 Enterococcus faecalis