Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reed, W.D.; Clinkenbeard, K.D.; Lane, M.D.
    Molecular and catalytic properties of mitochondrial (ketogenic) 3-hydroxy-3-methylglutaryl coenzyme A synthase of liver (1975), J. Biol. Chem., 250, 3117-3123.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 acetoacetyl-CoA
-
 Gallus gallus
2.3.3.10 acetoacetyl-CoA
-
 Rattus norvegicus
2.3.3.10 Mg2+ mitochondrial enzyme Gallus gallus
2.3.3.10 palmitoyl-CoA mitochondrial synthase Gallus gallus
2.3.3.10 succinyl-CoA mitochondrial synthase, 50% inhibition Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.005
-
 acetoacetyl-CoA
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 cytoplasm
-
 Rattus norvegicus 5737
-
2.3.3.10 cytoplasm distinct mitochondrial and cytoplasmic forms Gallus gallus 5737
-
2.3.3.10 cytosol
-
 Gallus gallus 5829
-
2.3.3.10 cytosol
-
 Rattus norvegicus 5829
-
2.3.3.10 mitochondrion
-
 Rattus norvegicus 5739
-
2.3.3.10 mitochondrion distinct mitochondrial and cytoplasmic forms Gallus gallus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.10 Mg2+ cytoplasmic enzyme Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 53000
-
 2 * 53000, SDS-PAGE Gallus gallus
2.3.3.10 57000
-
 2 * 57000, gel filtration Gallus gallus
2.3.3.10 96000
-
 gel filtration Gallus gallus
2.3.3.10 105000
-
 sedimentation equlibrium Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus mitochondrial enzyme of liver functions in ketogenesis, its cytoplasmic counterpart participates in cholesterogenesis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Gallus gallus
-
 chicken
-
2.3.3.10 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10 mitochondrial form Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.10 liver
-
 Gallus gallus
-
2.3.3.10 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 1
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.3.3.10 -20°C, purified synthase may be stored for 2-3 months with little loss of activity by adding 30% glyerol Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O mitochondrial enzyme of liver functions in ketogenesis, its cytoplasmic counterpart participates in cholesterogenesis Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.10 dimer 2 * 53000, SDS-PAGE Gallus gallus
2.3.3.10 dimer 2 * 57000, gel filtration Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.10 0.0005
-
 palmityl-CoA
-
 Gallus gallus