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Literature summary extracted from

  • Middleton, B.; Tubbs, P.K.
    3-Hydroxy-3-methylglutaryl-CoA synthase from baker's yeast (1975), Methods Enzymol., 35 B, 173-177.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.3.10 enzyme is labile to freezing and thawing, stabilized by glycerol Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 acetoacetyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 Cd2+
-
 Saccharomyces cerevisiae
2.3.3.10 CoA
-
 Saccharomyces cerevisiae
2.3.3.10 iodoacetamide
-
 Saccharomyces cerevisiae
2.3.3.10 iodoacetate
-
 Saccharomyces cerevisiae
2.3.3.10 N-ethylmaleimide
-
 Saccharomyces cerevisiae
2.3.3.10 p-chloromercuribenzoate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.003
-
 acetoacetyl-CoA pH 8.9 Saccharomyces cerevisiae
2.3.3.10 0.014
-
 acetyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 0.018
-
 acetyl-CoA
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 130000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 2.1
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.3.3.10 -20°, stored in presence of dithiothreitol and 30-50% glycerol purified enzyme retains complete activity for up to 3 months Saccharomyces cerevisiae
2.3.3.10 4°C, enzyme loses activity in absence of glycerol Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-3'-dephospho-CoA + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae 3-hydroxy-3-methylglutaryl-CoA + 3'-dephospho-CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-ACP + H2O
-
 Saccharomyces cerevisiae 3-hydroxy-3-methylglutaryl-ACP
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetylglutathione + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
2.3.3.10 acetylpantetheine + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae 3-hydroxy-3-methylglutaryl-CoA + pantetheine
-
 ?
2.3.3.10 additional information absolutely specific for the acyl-moiety of both its substrates, neither propionyl- nor butyryl CoA can replace acetyl-CoA Saccharomyces cerevisiae ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.10 0.02
-
 acetoacetyl-CoA pH 8.9 Saccharomyces cerevisiae