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Literature summary extracted from

  • Middleton, B.; Tubbs, P.K.
    The purification and some properties of 3-hydroxy-3-methylglutaryl-coenzyme A synthase from baker's yeast (1972), Biochem. J., 126, 27-34.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.3.3.10 enzyme is labile to freezing and thawing, stabilized by glycerol Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 5,5'-dithiobis(2-nitrobenzoate) residual activity 51% Saccharomyces cerevisiae
2.3.3.10 arsenite in presence of CoA, residual activity 25% Saccharomyces cerevisiae
2.3.3.10 bromoacetyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 CdSO4 residual activity 9% Saccharomyces cerevisiae
2.3.3.10 iodoacetamide residual activity 84% Saccharomyces cerevisiae
2.3.3.10 additional information desulfo-CoA protects against bromoacetyl-CoA inhibition, but not against p-chloromercuribenzoate and N-ethylmaleimide Saccharomyces cerevisiae
2.3.3.10 N-ethylmaleimide residual activity 46% Saccharomyces cerevisiae
2.3.3.10 NaAsO2 residual activity 90% Saccharomyces cerevisiae
2.3.3.10 p-chloromercuribenzoate residual activity 16% Saccharomyces cerevisiae
2.3.3.10 phenyl arsenious oxide in presence of CoA, residual activity 41%; residual activity 75% Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 130000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae synthesis of isoprenes and steroids (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 2.1
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O synthesis of isoprenes and steroids Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 additional information acetyl-CoA analogues can act as substrates, acetoacyl-analogues are no substrates Saccharomyces cerevisiae ?
-
 ?