EC Number | General Stability | Organism |
---|---|---|
2.3.3.10 | enzyme is labile to freezing and thawing, stabilized by glycerol | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.10 | 5,5'-dithiobis(2-nitrobenzoate) | residual activity 51% | Saccharomyces cerevisiae | |
2.3.3.10 | arsenite | in presence of CoA, residual activity 25% | Saccharomyces cerevisiae | |
2.3.3.10 | bromoacetyl-CoA | - |
Saccharomyces cerevisiae | |
2.3.3.10 | CdSO4 | residual activity 9% | Saccharomyces cerevisiae | |
2.3.3.10 | iodoacetamide | residual activity 84% | Saccharomyces cerevisiae | |
2.3.3.10 | additional information | desulfo-CoA protects against bromoacetyl-CoA inhibition, but not against p-chloromercuribenzoate and N-ethylmaleimide | Saccharomyces cerevisiae | |
2.3.3.10 | N-ethylmaleimide | residual activity 46% | Saccharomyces cerevisiae | |
2.3.3.10 | NaAsO2 | residual activity 90% | Saccharomyces cerevisiae | |
2.3.3.10 | p-chloromercuribenzoate | residual activity 16% | Saccharomyces cerevisiae | |
2.3.3.10 | phenyl arsenious oxide | in presence of CoA, residual activity 41%; residual activity 75% | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.10 | 130000 | - |
gel filtration | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.10 | acetyl-CoA + acetoacetyl-CoA + H2O | Saccharomyces cerevisiae | synthesis of isoprenes and steroids | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.10 | Saccharomyces cerevisiae | - |
baker's yeast | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.10 | - |
Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.3.10 | 2.1 | - |
- |
Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.10 | acetyl-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
2.3.3.10 | acetyl-CoA + acetoacetyl-CoA + H2O | synthesis of isoprenes and steroids | Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
2.3.3.10 | additional information | acetyl-CoA analogues can act as substrates, acetoacyl-analogues are no substrates | Saccharomyces cerevisiae | ? | - |
? |