EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.16 | expression of chimeric mutant in Escherichia coli citrate synthase deficient strain MOB154 | Thermoplasma acidophilum |
2.3.3.16 | expression of wild-type, chimeric mutant and site-directed mutants in Escherichia coli citrate synthase deficient strain MOB154 | Pyrococcus furiosus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.3.16 | crystal structure analysis of chimeric mutants with exchange of large and small subunits between Thermoplasma acidophilum and Pyrococcus furiosus | Pyrococcus furiosus |
2.3.3.16 | crystal structure analysis of chimeric mutants with exchange of large and small subunits between Thermoplasma acidophilum and Pyrococcus furiosus | Thermoplasma acidophilum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.3.16 | D113A | site-directed mutagenesis, deletion of C-terminal amino acid residues which arrange the subunit contact, slightly increased Km for acetyl-CoA and oxaloacetate, slightly increased reaction velocity, reduced thermostability | Pyrococcus furiosus |
2.3.3.16 | D113S | site-directed mutagenesis, deletion of C-terminal amino acid residues which arrange the subunit contact, slightly decreased Km for acetyl-CoA and oxaloacetate, slightly increased reaction velocity, reduced thermostability | Pyrococcus furiosus |
2.3.3.16 | additional information | construction of mutant with disrupted inter-subunit ionic network by partly eleminating the C-terminal end amino acid residues, increased Km for substrates, reduced thermostability | Pyrococcus furiosus |
2.3.3.16 | additional information | construction of chimeric enzyme mutants with mix of the large and small subunits of Thermoplasma acidophilum and Pyrococcus furiosus, functional analysis of the subunits | Pyrococcus furiosus |
2.3.3.16 | additional information | construction of chimeric enzyme mutants with mix of the large and small subunits of Thermoplasma acidophilum and Pyrococcus furiosus, functional analysis of the subunits | Thermoplasma acidophilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.16 | 0.002 | - |
oxaloacetate | wild-type | Thermoplasma acidophilum | |
2.3.3.16 | 0.004 | - |
acetyl-CoA | wild-type | Pyrococcus furiosus | |
2.3.3.16 | 0.005 | - |
acetyl-CoA | wild-type | Thermoplasma acidophilum | |
2.3.3.16 | 0.015 | - |
oxaloacetate | wild-type | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.16 | Pyrococcus furiosus | - |
- |
- |
2.3.3.16 | Thermoplasma acidophilum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.16 | recombinant chimeric mutants from E. coli | Thermoplasma acidophilum |
2.3.3.16 | recombinant wild-type, chimeric mutants, C-terminal deletion mutants, and D113 mutants from E. coli | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.16 | acetyl-CoA + oxaloacetate + H2O | - |
Pyrococcus furiosus | citrate + CoA | - |
? | |
2.3.3.16 | acetyl-CoA + oxaloacetate + H2O | - |
Thermoplasma acidophilum | citrate + CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.3.16 | dimer | - |
Pyrococcus furiosus |
2.3.3.16 | dimer | - |
Thermoplasma acidophilum |
2.3.3.16 | More | chimeric mutants with exchanged large and small subunits between Thermoplasma acidophilum and Pyrococcus furiosus, the large subunit is responsible for subunit interaction, the small subunit is responsable for catalytic activity | Pyrococcus furiosus |
2.3.3.16 | More | chimeric mutants with exchanged large and small subunits between Thermoplasma acidophilum and Pyrococcus furiosus, the large subunit is responsible for subunit interaction, the small subunit is responsable for catalytic activity | Thermoplasma acidophilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | 65 | - |
enzyme mutant lacking 13 amino acid residues of the C-terminal | Pyrococcus furiosus |
2.3.3.16 | 65 | 70 | chimeric mutant containing the large subunit of Pyrcoccus furiosus and the small subunit of Thermoplasma acidophilum | Pyrococcus furiosus |
2.3.3.16 | 65 | 70 | chimeric mutant containing the large subunit of Pyrcoccus furiosus and the small subunit of Thermoplasma acidophilum | Thermoplasma acidophilum |
2.3.3.16 | 70 | 75 | wild-type | Thermoplasma acidophilum |
2.3.3.16 | 75 | - |
enzyme mutant lacking 2 amino acid residues of the C-terminal | Pyrococcus furiosus |
2.3.3.16 | 80 | - |
mutants D113S and D113A | Pyrococcus furiosus |
2.3.3.16 | 90 | - |
above, wild-type | Pyrococcus furiosus |
2.3.3.16 | 90 | - |
chimeric mutant containing the large subunit of Thermoplasma acidophilum and the small subunit of Pyrcoccus furiosus | Pyrococcus furiosus |
2.3.3.16 | 90 | - |
chimeric mutant containing the large subunit of Thermoplasma acidophilum and the small subunit of Pyrcoccus furiosus | Thermoplasma acidophilum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | 40 | 90 | - |
Pyrococcus furiosus |
2.3.3.16 | 40 | 90 | - |
Thermoplasma acidophilum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | additional information | - |
thermostability of wild-type and chimeric mutants | Pyrococcus furiosus |
2.3.3.16 | additional information | - |
thermostability of wild-type and chimeric mutants | Thermoplasma acidophilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | 8 | - |
enzyme assay at | Pyrococcus furiosus |
2.3.3.16 | 8 | - |
enzyme assay at | Thermoplasma acidophilum |