EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.16 | expression of wild-type and mutant in Escherichia coli | Thermoplasma acidophilum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.3.16 | S43C | site-directed mutagenesis, 5.7fold reduced activity, unaltered Km values for the substrates and unaltered thermostability | Thermoplasma acidophilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.16 | 0.01 | - |
oxaloacetate | recombinant wild-type and mutant S34C | Thermoplasma acidophilum | |
2.3.3.16 | 0.011 | - |
acetyl-CoA | recombinant wild-type and mutant S43C | Thermoplasma acidophilum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.16 | Thermoplasma acidophilum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.16 | native and recombinant wild-type, the latter from E. coli and recombinant S43C mutant enzyme from E. coli | Thermoplasma acidophilum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | 9 | - |
S43C mutant | Thermoplasma acidophilum |
2.3.3.16 | 51.4 | - |
wild-type | Thermoplasma acidophilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.16 | acetyl-CoA + oxaloacetate + H2O | - |
Thermoplasma acidophilum | citrate + CoA | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | additional information | - |
effect of C43 mutation | Thermoplasma acidophilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.16 | 8 | - |
recombinant wild-type and mutant | Thermoplasma acidophilum |