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Literature summary extracted from

  • Rault-Leonardon, M.; Atkinson, M.A.L.; Slaughter, C.A.; Moomaw, C.R.; Srere, P.A.
    Azotobacter vinelandii citrate synthase (1995), Biochemistry, 34, 257-263.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 NADH 70% inhibition at 2 mM, completely reversed by 0.17 mM 5'-AMP Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 additional information
-
 additional information allosteric, Hill coefficient: 1.5 Azotobacter vinelandii
2.3.3.16 0.014
-
 acetyl-CoA with 0.1 M KCl Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.16 KCl
-
 Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 additional information
-
 alignment of partial amino acid sequence Azotobacter vinelandii
2.3.3.16 48000
-
 6 * 48000, SDS-PAGE Azotobacter vinelandii
2.3.3.16 249000 275000 equilibrium sedimentation Azotobacter vinelandii
2.3.3.16 330000
-
 gel filtration Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Azotobacter vinelandii high activity necessary for N2 fixation citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Azotobacter vinelandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 partial Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Azotobacter vinelandii citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O high activity necessary for N2 fixation Azotobacter vinelandii citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 hexamer 6 * 48000, SDS-PAGE Azotobacter vinelandii

Synonyms

EC Number Synonyms Comment Organism
2.3.3.16 CS
-
 Azotobacter vinelandii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 25
-
 enzyme assay at Azotobacter vinelandii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.16 111
-
 oxaloacetate
-
 Azotobacter vinelandii
2.3.3.16 170
-
 oxaloacetate with 0.1 mM KCl Azotobacter vinelandii
2.3.3.16 174
-
 acetyl-CoA with 0.1 M KCl Azotobacter vinelandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 7.5
-
 enzyme assay at Azotobacter vinelandii

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.3.16 AMP positive effector Azotobacter vinelandii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.16 0.012
-
 NADH in 20 mM Tris-HCl Azotobacter vinelandii
2.3.3.16 0.66
-
 NADH in 0.1 M Tris-HCl Azotobacter vinelandii