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Literature summary extracted from

  • Nakayama, R.; Kumagai, H.; Tochikura, T.
    Purification and properties of gamma-glutamyltranspeptidase from Proteus mirabilis (1984), J. Bacteriol., 160, 341-346.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.2 ammonium sulfate precipitation, refrigerator, 2 weeks Proteus mirabilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 6-diazo-5-oxo-L-norleucine
-
Proteus mirabilis
2.3.2.2 Aminooxyacetate hydrolase reaction Proteus mirabilis
2.3.2.2 beta-chloro-L-alanine
-
Proteus mirabilis
2.3.2.2 glutathione no inhibition Proteus mirabilis
2.3.2.2 glycine weak Proteus mirabilis
2.3.2.2 Hg2+
-
Proteus mirabilis
2.3.2.2 iodoacetamide
-
Proteus mirabilis
2.3.2.2 iodoacetate weak Proteus mirabilis
2.3.2.2 L- or D-Serine/borate L-serine/borate, not D-serine/borate Proteus mirabilis
2.3.2.2 L-alanine
-
Proteus mirabilis
2.3.2.2 L-glutamine not D- Proteus mirabilis
2.3.2.2 additional information inhibition by diverse amino acids, overview Proteus mirabilis
2.3.2.2 N-ethylmaleimide no inhibition Proteus mirabilis
2.3.2.2 p-chloromercuribenzoate no inhibition Proteus mirabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.033
-
glutathione hydrolase reaction Proteus mirabilis
2.3.2.2 0.13 0.18 L-Glu-4-nitroanilide with GSH Proteus mirabilis
2.3.2.2 0.18
-
glutathione transpeptidation Proteus mirabilis
2.3.2.2 0.31
-
L-Glu-4-nitroanilide hydrolase reaction Proteus mirabilis
2.3.2.2 0.4
-
L-Glu-4-nitroanilide transpeptidation Proteus mirabilis
2.3.2.2 2.9 2.96 glycylglycine
-
Proteus mirabilis
2.3.2.2 10
-
L-glutamine hydrolase reaction Proteus mirabilis
2.3.2.2 32
-
L-Phe
-
Proteus mirabilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 28000
-
1 * 47000 + 1 * 28000, SDS-PAGE Proteus mirabilis
2.3.2.2 47000
-
1 * 47000 + 1 * 28000, SDS-PAGE Proteus mirabilis
2.3.2.2 80000
-
gel filtration Proteus mirabilis

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Proteus mirabilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
Proteus mirabilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 16
-
purified enzyme Proteus mirabilis

Storage Stability

EC Number Storage Stability Organism
2.3.2.2 4°C, crystalline, at least 3 months Proteus mirabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Proteus mirabilis peptide + 5-L-glutamyl amino acid
-
?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Proteus mirabilis peptide + 5-L-glutamyl amino acid
-
?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Proteus mirabilis peptide + 5-L-glutamyl amino acid
-
?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Proteus mirabilis peptide + 5-L-glutamyl amino acid
-
?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor specificity, overview Proteus mirabilis peptide + 5-L-glutamyl amino acid
-
?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
Proteus mirabilis 4-nitroaniline + 5-L-glutamylglycylglycine
-
?
2.3.2.2 GSH + L-glutamine
-
Proteus mirabilis ?
-
?
2.3.2.2 GSH + L-Phe
-
Proteus mirabilis ?
-
?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
Proteus mirabilis 4-nitroaniline + 5-L-glutamyl-glutathione
-
?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 47000 + 1 * 28000, SDS-PAGE Proteus mirabilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
assay at Proteus mirabilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.2 40
-
10% loss of activity within 10 min Proteus mirabilis
2.3.2.2 45
-
37% loss of activity within 10 min Proteus mirabilis
2.3.2.2 50
-
95% loss of activity within 10 min Proteus mirabilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 6
-
hydrolase reaction Proteus mirabilis
2.3.2.2 7.5 8 transpeptidation Proteus mirabilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.2.2 4
-
50% loss of activity after 60 min, 37°C Proteus mirabilis
2.3.2.2 5 9 at least 60 min stable, 37°C Proteus mirabilis
2.3.2.2 10
-
50% loss of activity after 60 min, 37°C Proteus mirabilis