EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.2.15 | 1.5 | - |
monobromobimane-glutathione | - |
Silene vulgaris | |
2.3.2.15 | 6.7 | - |
glutathione | - |
Silene vulgaris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.2.15 | Ag+ | activation | Silene vulgaris | |
2.3.2.15 | Bi3+ | activation | Silene vulgaris | |
2.3.2.15 | Cd2+ | activation | Silene vulgaris | |
2.3.2.15 | Cu2+ | activation | Silene vulgaris | |
2.3.2.15 | Hg2+ | activation | Silene vulgaris | |
2.3.2.15 | Pb2+ | activation | Silene vulgaris | |
2.3.2.15 | Zn2+ | activation | Silene vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.2.15 | 25000 | - |
4 * 25000, SDS-PAGE, dimer also catalytically active | Silene vulgaris |
2.3.2.15 | 95000 | - |
gel filtration | Silene vulgaris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.15 | glutathione + [Glu(-Cys)]n-Gly | Podophyllum peltatum | n = 2-5 | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
2.3.2.15 | glutathione + [Glu(-Cys)]n-Gly | Silene vulgaris | critical for heavy metal tolerance | Gly + [Glu(-Cys)]n+1-Gly | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.15 | Beta vulgaris | - |
- |
- |
2.3.2.15 | Equisetum giganteum | - |
- |
- |
2.3.2.15 | Eschscholzia californica | - |
- |
- |
2.3.2.15 | Podophyllum peltatum | - |
- |
- |
2.3.2.15 | Silene vulgaris | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.2.15 | - |
Silene vulgaris |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.2.15 | -20°C, 10 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol, 0.1% NaN3 | Silene vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.15 | glutathione + [Glu(-Cys)]n-Gly | - |
Beta vulgaris | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
2.3.2.15 | glutathione + [Glu(-Cys)]n-Gly | - |
Eschscholzia californica | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
2.3.2.15 | glutathione + [Glu(-Cys)]n-Gly | - |
Silene vulgaris | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
2.3.2.15 | glutathione + [Glu(-Cys)]n-Gly | - |
Equisetum giganteum | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
2.3.2.15 | glutathione + [Glu(-Cys)]n-Gly | - |
Podophyllum peltatum | Gly + [Glu(-Cys)]n+1-Gly | n = 2-5 | ? | |
2.3.2.15 | glutathione + [Glu(-Cys)]n-Gly | n = 2-5 | Podophyllum peltatum | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
2.3.2.15 | glutathione + [Glu(-Cys)]n-Gly | critical for heavy metal tolerance | Silene vulgaris | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
2.3.2.15 | monobromobimane-glutathione + [Glu(-Cys)]n-Gly | - |
Silene vulgaris | Gly + [Glu(-Cys)]n-Glu-S-monobromobimane-Cys-Gly | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.2.15 | homotetramer | 4 * 25000, SDS-PAGE, dimer also catalytically active | Silene vulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.2.15 | 35 | - |
- |
Silene vulgaris |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.2.15 | 20 | 47 | half-maximal activity at 20°C and 47°C | Silene vulgaris |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.2.15 | -20 | - |
30% glycerol, 500 h, small activity decrease | Silene vulgaris |
2.3.2.15 | 4 | - |
50% activity after 140 h | Silene vulgaris |
2.3.2.15 | 22 | - |
50% activity after 34 h | Silene vulgaris |
2.3.2.15 | 35 | - |
50% activity after 0.5 h | Silene vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.2.15 | 7.9 | - |
- |
Silene vulgaris |