Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Grill, E.; Löffler, S.; Winnacker, E.L.; Zenk, M.H.
    Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific gamma-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase) (1989), Proc. Natl. Acad. Sci. USA, 86, 6838-6842.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.15 1.5
-
monobromobimane-glutathione
-
Silene vulgaris
2.3.2.15 6.7
-
glutathione
-
Silene vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.15 Ag+ activation Silene vulgaris
2.3.2.15 Bi3+ activation Silene vulgaris
2.3.2.15 Cd2+ activation Silene vulgaris
2.3.2.15 Cu2+ activation Silene vulgaris
2.3.2.15 Hg2+ activation Silene vulgaris
2.3.2.15 Pb2+ activation Silene vulgaris
2.3.2.15 Zn2+ activation Silene vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.15 25000
-
4 * 25000, SDS-PAGE, dimer also catalytically active Silene vulgaris
2.3.2.15 95000
-
gel filtration Silene vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly Podophyllum peltatum n = 2-5 Gly + [Glu(-Cys)]n+1-Gly
-
?
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly Silene vulgaris critical for heavy metal tolerance Gly + [Glu(-Cys)]n+1-Gly
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.15 Beta vulgaris
-
-
-
2.3.2.15 Equisetum giganteum
-
-
-
2.3.2.15 Eschscholzia californica
-
-
-
2.3.2.15 Podophyllum peltatum
-
-
-
2.3.2.15 Silene vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.15
-
Silene vulgaris

Storage Stability

EC Number Storage Stability Organism
2.3.2.15 -20°C, 10 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol, 0.1% NaN3 Silene vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
Beta vulgaris Gly + [Glu(-Cys)]n+1-Gly
-
?
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
Eschscholzia californica Gly + [Glu(-Cys)]n+1-Gly
-
?
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
Silene vulgaris Gly + [Glu(-Cys)]n+1-Gly
-
?
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
Equisetum giganteum Gly + [Glu(-Cys)]n+1-Gly
-
?
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
Podophyllum peltatum Gly + [Glu(-Cys)]n+1-Gly n = 2-5 ?
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly n = 2-5 Podophyllum peltatum Gly + [Glu(-Cys)]n+1-Gly
-
?
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly critical for heavy metal tolerance Silene vulgaris Gly + [Glu(-Cys)]n+1-Gly
-
?
2.3.2.15 monobromobimane-glutathione + [Glu(-Cys)]n-Gly
-
Silene vulgaris Gly + [Glu(-Cys)]n-Glu-S-monobromobimane-Cys-Gly
-
?

Subunits

EC Number Subunits Comment Organism
2.3.2.15 homotetramer 4 * 25000, SDS-PAGE, dimer also catalytically active Silene vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.15 35
-
-
Silene vulgaris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.2.15 20 47 half-maximal activity at 20°C and 47°C Silene vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.15 -20
-
30% glycerol, 500 h, small activity decrease Silene vulgaris
2.3.2.15 4
-
50% activity after 140 h Silene vulgaris
2.3.2.15 22
-
50% activity after 34 h Silene vulgaris
2.3.2.15 35
-
50% activity after 0.5 h Silene vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.15 7.9
-
-
Silene vulgaris