BRENDA - Enzyme Database

Properties of purified lens transglutaminase and regulation of its transamidase/crosslinking activity by GTP

Murthy, S.N.P.; Velasco, P.T.; Lorand, L.; Exp. Eye Res. 67, 273-281 (1998)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.2.13
dithiothreitol
-
Cavia porcellus
2.3.2.13
dithiothreitol
required for activity of lens transglutaminase
Oryctolagus cuniculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.2.13
GTP
-
Cavia porcellus
2.3.2.13
GTP
0.05 mM, 50% inhibition of lens transglutaminase, 0.5 mM, complete inhibition in the presence of 0.5 mM Ca2+, increasing the Ca2+ concentration to 3 mM reverses inhibition
Oryctolagus cuniculus
2.3.2.13
GTP
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.2.13
Ca2+
requirement
Cavia porcellus
2.3.2.13
Ca2+
requirement
Homo sapiens
2.3.2.13
Ca2+
requirement
Oryctolagus cuniculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.2.13
78000
-
x * 78000, lens transglutaminase, SDS-PAGE
Oryctolagus cuniculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.2.13
Cavia porcellus
-
-
-
2.3.2.13
Homo sapiens
-
-
-
2.3.2.13
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.2.13
lens transglutaminase, DE-52, affinity chromatography on 42000 Da thermolytic fragment of human plasma fibronectin
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.2.13
lens
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.2.13
monodansylcadaverine + N,N-dimethylcasein
-
487867
Oryctolagus cuniculus
?
-
-
-
?
2.3.2.13
protein-bound gamma-glutamine + alkylamine
-
487867
Cavia porcellus
protein N5-alkylglutamine + NH3
-
487867
Cavia porcellus
-
2.3.2.13
protein-bound gamma-glutamine + alkylamine
-
487867
Homo sapiens
protein N5-alkylglutamine + NH3
-
487867
Homo sapiens
-
2.3.2.13
protein-bound gamma-glutamine + alkylamine
lens transglutaminase, crosslinking of beta-crystallin
487867
Oryctolagus cuniculus
protein N5-alkylglutamine + NH3
-
487867
Oryctolagus cuniculus
-
Subunits
EC Number
Subunits
Commentary
Organism
2.3.2.13
?
-
Homo sapiens
2.3.2.13
?
x * 78000, lens transglutaminase, SDS-PAGE
Oryctolagus cuniculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.2.13
dithiothreitol
-
Cavia porcellus
2.3.2.13
dithiothreitol
required for activity of lens transglutaminase
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.2.13
GTP
-
Cavia porcellus
2.3.2.13
GTP
0.05 mM, 50% inhibition of lens transglutaminase, 0.5 mM, complete inhibition in the presence of 0.5 mM Ca2+, increasing the Ca2+ concentration to 3 mM reverses inhibition
Oryctolagus cuniculus
2.3.2.13
GTP
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.2.13
Ca2+
requirement
Cavia porcellus
2.3.2.13
Ca2+
requirement
Homo sapiens
2.3.2.13
Ca2+
requirement
Oryctolagus cuniculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.2.13
78000
-
x * 78000, lens transglutaminase, SDS-PAGE
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.2.13
lens transglutaminase, DE-52, affinity chromatography on 42000 Da thermolytic fragment of human plasma fibronectin
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.2.13
lens
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.2.13
monodansylcadaverine + N,N-dimethylcasein
-
487867
Oryctolagus cuniculus
?
-
-
-
?
2.3.2.13
protein-bound gamma-glutamine + alkylamine
-
487867
Cavia porcellus
protein N5-alkylglutamine + NH3
-
487867
Cavia porcellus
-
2.3.2.13
protein-bound gamma-glutamine + alkylamine
-
487867
Homo sapiens
protein N5-alkylglutamine + NH3
-
487867
Homo sapiens
-
2.3.2.13
protein-bound gamma-glutamine + alkylamine
lens transglutaminase, crosslinking of beta-crystallin
487867
Oryctolagus cuniculus
protein N5-alkylglutamine + NH3
-
487867
Oryctolagus cuniculus
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.2.13
?
-
Homo sapiens
2.3.2.13
?
x * 78000, lens transglutaminase, SDS-PAGE
Oryctolagus cuniculus