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Literature summary extracted from

  • Mottahedeh, J.; Marsh, R.
    Characterization of 101-kDa transglutaminase from Physarum polycephalum and identification of LAV1-2 as substrate (1998), J. Biol. Chem., 273, 29888-29895.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 KCl 45% activity at 400 mM Physarum polycephalum
2.3.2.13 Monodansylcadaverine partial inhibition above 0.5 mM Physarum polycephalum
2.3.2.13 N-ethylmaleimide irreversible inhibition, inhibition increases with increasing Ca2+ concentrations, 50% inhibition at 1 mM Ca2+ Physarum polycephalum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 0.034
-
 Monodansylcadaverine
-
 Physarum polycephalum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ requirement Physarum polycephalum
2.3.2.13 Ca2+ half-maximal activity at 0.7 mM, maximal activity at about 2 mM Physarum polycephalum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 96000
-
 1 * 96000, gel filtration in the presence of 1 mM Ca2+ Physarum polycephalum
2.3.2.13 101000
-
 gel filtration Physarum polycephalum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 protein-bound gamma-glutamine + alkylamine Physarum polycephalum plasmodia-specific 40000 Da protein LAV1-2 is the preferred in situ substrate protein N5-alkylglutamine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Physarum polycephalum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 streptomycin sulfate, DEAE-cellulose, phenyl-Sepharose Physarum polycephalum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.13 8.8
-
 incorporation of monodansylcadaverine into N,N-dimethylcasein Physarum polycephalum

Storage Stability

EC Number Storage Stability Organism
2.3.2.13 -70°C, 6 months, little loss in activity Physarum polycephalum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 monodansylcadaverine + N,N-dimethylcasein
-
 Physarum polycephalum ?
-
 ?
2.3.2.13 protein-bound gamma-glutamine + alkylamine plasmodia-specific 40000 Da protein LAV1-2 is the preferred in situ substrate Physarum polycephalum protein N5-alkylglutamine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 monomer 1 * 96000, gel filtration in the presence of 1 mM Ca2+ Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.13 7.5
-
 less than 50% of maximal activity at pH 5,5 and pH 9.5 Physarum polycephalum