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Literature summary extracted from

  • Kumazawa, Y.; Ohtsuka, T.; Ninomiya, D.; Seguro, K.
    Purification and calcium dependence of transglutaminases from sheep hair follicles (1997), Biosci. Biotechnol. Biochem., 61, 1086-1090.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 iodoacetamide 1 mM, 89% inhibition of DEAE-absorbed transglutaminase Ovis aries
2.3.2.13 lysine 1 mM, 43% inhibition of DEAE-unabsorbed transglutaminase, 90% inhibition of DEAE-absorbed transglutaminase Ovis aries
2.3.2.13 monoiodoacetate 1 mM, 93% inhibition of DEAE-unabsorbed transglutaminase, 85% inhibition of DEAE-absorbed transglutaminase Ovis aries
2.3.2.13 N-ethylmaleimide 1 mM, complete inhibition of DEAE-unabsorbed transglutaminase, 93% inhibition of DEAE-absorbed transglutaminase Ovis aries

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ requirement Ovis aries

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 40000
-
 2 * 40000, DEAE-absorbed transglutaminase, SDS-PAGE Ovis aries
2.3.2.13 77000
-
 1 * 77000, DEAE-unabsorbed transglutaminase, SDS-PAGE Ovis aries
2.3.2.13 80000
-
 DEAE-absorbed transglutaminase, gel filtration Ovis aries
2.3.2.13 82000
-
 DEAE-unabsorbed transglutaminase, gel filtration Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Ovis aries
-
 Merino sheep
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 DEAE-unabsorbed transglutaminase, CM-Sepharose, heparin-Sepharose, Superdex 200, DEAE-absorbed transglutaminase, DEAE-Sephacel, heparin-Sepharose, hydroxyapatite, Superdex 200 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.13 hair follicle
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.13 0.0052
-
 DEAE-unabsorbed transglutaminase Ovis aries
2.3.2.13 0.092
-
 DEAE-absorbed transglutaminase Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 protein-bound gamma-glutamine + alkylamine crosslinking of Hammersten casein, crosslinking between Ac-IB and Bz-Gly-Lys Ovis aries protein N5-alkylglutamine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 dimer 2 * 40000, DEAE-absorbed transglutaminase, SDS-PAGE Ovis aries
2.3.2.13 monomer 1 * 77000, DEAE-unabsorbed transglutaminase, SDS-PAGE Ovis aries

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.13 60
-
-
 Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.13 8.5 9
-
 Ovis aries