Literature summary extracted from
Tokunaga, F.; Yamada, M.; Miyata, T.; Ding, Y.L.; Hiranaga-Kawabata, M.; Muta, T.; Iwanaga, S.
Limulus hemocyte transglutaminase. Its purification and characterization, and identification of the intracellular substrates (1993), J. Biol. Chem., 268, 252-261.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.3.2.13 |
dithiothreitol |
weak activation |
Tachypleus tridentatus |
|
General Stability
EC Number |
General Stability |
Organism |
---|
2.3.2.13 |
loss of activity after freezing |
Tachypleus tridentatus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.3.2.13 |
Cu2+ |
1 mM, complete inhibition |
Tachypleus tridentatus |
|
2.3.2.13 |
EDTA |
5 mM; 5 mM, complete inhibition |
Tachypleus tridentatus |
|
2.3.2.13 |
hydroxylamine |
100 mM, complete inhibition |
Tachypleus tridentatus |
|
2.3.2.13 |
KCl |
above 500 mM |
Tachypleus tridentatus |
|
2.3.2.13 |
monoiodoacetate |
1 mM, 94% inhibition |
Tachypleus tridentatus |
|
2.3.2.13 |
additional information |
not inhibited by diisopropylfluorophosphate |
Tachypleus tridentatus |
|
2.3.2.13 |
N-ethylmaleimide |
1 mM, 98% inhibition |
Tachypleus tridentatus |
|
2.3.2.13 |
NaCl |
above 500 mM |
Tachypleus tridentatus |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.3.2.13 |
soluble |
- |
Tachypleus tridentatus |
- |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.3.2.13 |
Ca2+ |
requirement |
Tachypleus tridentatus |
|
2.3.2.13 |
Ca2+ |
8 mM, approx. 10fold activation |
Tachypleus tridentatus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.3.2.13 |
86000 |
- |
1 * 86000, SDS-PAGE |
Tachypleus tridentatus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.2.13 |
Tachypleus tridentatus |
- |
japanese horseshoe crab |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.3.2.13 |
CM-Sepharose CL-6B, ammonium sulfate, DEAE-cellulose, Sephacryl S-300, DEAE-cosmogel, Zn-chelating Sepharose |
Tachypleus tridentatus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
2.3.2.13 |
hemocyte |
- |
Tachypleus tridentatus |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.3.2.13 |
additional information |
- |
938 amine incorporation units/min |
Tachypleus tridentatus |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.3.2.13 |
4°C, at least 3 months |
Tachypleus tridentatus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.2.13 |
protein-bound gamma-glutamine + alkylamine |
identification of natural protein substrates |
Tachypleus tridentatus |
protein N5-alkylglutamine + NH3 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.3.2.13 |
monomer |
1 * 86000, SDS-PAGE |
Tachypleus tridentatus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.3.2.13 |
37 |
- |
assay at |
Tachypleus tridentatus |