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Literature summary extracted from

  • Rhodes, M.J.C.; Wooltorton, L.S.C.; Lourencq, E.J.
    Purification and properties of hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase from potatoes (1979), Phytochemistry, 18, 1125-1129.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.99 bovine serum albumin activation Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.99 caffeic acid
-
 Solanum tuberosum
2.3.1.99 Cinnamic acid
-
 Solanum tuberosum
2.3.1.99 coumaric acid
-
 Solanum tuberosum
2.3.1.99 ferulic acid
-
 Solanum tuberosum
2.3.1.99 Hg2+
-
 Solanum tuberosum
2.3.1.99 additional information not: shikimate; very slight inhibition by Mg2+, Be2+, Ca2+, Cd2+ Solanum tuberosum
2.3.1.99 quinic acid
-
 Solanum tuberosum
2.3.1.99 Tris/HCl above 10 mM Solanum tuberosum
2.3.1.99 Zn2+
-
 Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.99 0.021
-
 CoA
-
 Solanum tuberosum
2.3.1.99 0.073
-
 caffeoyl-CoA
-
 Solanum tuberosum
2.3.1.99 0.151
-
 chlorogenic acid
-
 Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.99 14900
-
 3 * 14900, SDS-PAGE Solanum tuberosum
2.3.1.99 41500
-
 gel filtration Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.99 additional information Solanum tuberosum enzyme plays a role in the endergonic processes involved in the synthetic reactions leading to esterification of quinic acid with hydroxycinnamoyl residues ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.99 Solanum tuberosum
-
 potato, var. Homeguard
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.99
-
 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.99 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.99 16.74
-
-
 Solanum tuberosum

Storage Stability

EC Number Storage Stability Organism
2.3.1.99 -20°C, 33% loss of activity after 10 months Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.99 4-coumaroyl-CoA + quinate best substrate Solanum tuberosum CoA + 5-O-(4-coumaroyl)quinate
-
 r
2.3.1.99 caffeoyl-CoA + quinate
-
 Solanum tuberosum CoA + 5-O-caffeoylquinate i.e. chlorogenic acid r
2.3.1.99 caffeoyl-CoA + quinate
-
 Solanum tuberosum CoA + chlorogenic acid
-
 ?
2.3.1.99 feruloyl-CoA + quinate
-
 Solanum tuberosum CoA + O-feruloylquinate
-
 r
2.3.1.99 additional information enzyme plays a role in the endergonic processes involved in the synthetic reactions leading to esterification of quinic acid with hydroxycinnamoyl residues Solanum tuberosum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.99 trimer 3 * 14900, SDS-PAGE Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.99 50
-
 65% loss of activity after 5 min, phosphate buffer protects: 12% loss after 5 min Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.99 6.3
-
-
 Solanum tuberosum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.99 0.074
-
 Zn2+
-
 Solanum tuberosum
2.3.1.99 0.7
-
 coumaric acid
-
 Solanum tuberosum
2.3.1.99 0.82
-
 caffeic acid
-
 Solanum tuberosum
2.3.1.99 0.94
-
 Cinnamic acid
-
 Solanum tuberosum
2.3.1.99 1.1
-
 quinic acid
-
 Solanum tuberosum
2.3.1.99 1.17
-
 ferulic acid
-
 Solanum tuberosum