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Literature summary extracted from

  • Raju, R.V.S.; Kakkar, R.; Datla, R.S.S.; Radhi, J.; Sharma, R.K.
    Myristoyl-CoA:protein N-myristoyltransferase from bovine cardiac muscle: molecular cloning, kinetic analysis, and in vitro proteolytic cleavage by m-calpain (1998), Exp. Cell Res., 241, 23-35.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.97 cloning of cardiac muscle enzyme, expression in Escherichia coli, DNA and amino acid sequence determination Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.97 m-calpain enzyme is inactivated by cleavage, protease is specific for PEST regions, i.e. regions rich in proline, glutamic acid, serine and threonine, calpain-inhibitor calpastatin protects Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.97 0.0058
-
 myristoyl-CoA recombinant enzyme Bos taurus
2.3.1.97 0.016
-
 p60src-derived peptide recombinant enzyme Bos taurus
2.3.1.97 0.05
-
 M2 gene segment of reovirus type 3-derived peptide recombinant enzyme Bos taurus
2.3.1.97 0.1
-
 cAMP-dependent protein kinase-derived peptide recombinant enzyme Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.97 cytosol major part Bos taurus 5829
-
2.3.1.97 mitochondrion occasionally Bos taurus 5739
-
2.3.1.97 myofibril occasionally Bos taurus 30016
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.97 additional information
-
 amino acid sequence, alignment Bos taurus
2.3.1.97 50000
-
 1 * 50000, recombinant enzyme Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.97 myristoyl-CoA + glycylpeptide Bos taurus enzyme is possibly regulated by calpains in vivo N-myristoylglycylpeptide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.97 cardiac muscle enzyme, recombinant from Escherichia coli Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.97 tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein] PEST regions for recognition by clapains, putative regulatory enzymes Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.97 cardiac muscle cardiac, low activity Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 myristoyl-CoA + cAMP-dependent protein kinase-derived peptide
-
 Bos taurus N-myristoylated cAMP-dependent protein kinase-derived peptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg pp60src-derived peptide Bos taurus N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide substrate specificity Bos taurus N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide enzyme is possibly regulated by calpains in vivo Bos taurus N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + M2 gene segment of reovirus type 3-derived peptide
-
 Bos taurus N-myristoylated M2 gene segment of reovirus type 3-derived peptide + CoA
-
 ?
2.3.1.97 palmitoyl-CoA + glycylpeptide poor substrate Bos taurus N-palmitoylglycylpeptide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.97 monomer 1 * 50000, recombinant enzyme Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 NMT
-
 Bos taurus