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Literature summary extracted from

  • Suzuki, H.; Murakoshi, I.; Saito, K.
    A novel O-tigloyltransferase for alkaloid biosynthesis in plants. Purification, characterization, and distribution in Lupinus plants (1994), J. Biol. Chem., 269, 15853-15860.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.93 dithiothreitol
-
 Lupinus albus
2.3.1.93 dithiothreitol 140% activity at 1 mM Lupinus albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.93 (+)-epilupinine
-
 Lupinus albus
2.3.1.93 (+)-lupanine
-
 Lupinus albus
2.3.1.93 CoA competitive Lupinus albus
2.3.1.93 N-ethylmaleimide
-
 Lupinus albus
2.3.1.93 p-chloromercuriphenyl sulfonic acid
-
 Lupinus albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.93 0.021
-
 13-alpha-hydroxymultiflorine
-
 Lupinus albus
2.3.1.93 0.027
-
 13-hydroxylupanine
-
 Lupinus albus
2.3.1.93 0.46
-
 tigloyl-CoA with 13-alpha-hydroxymultiflorine Lupinus albus
2.3.1.93 0.52
-
 tigloyl-CoA with 13-alpha-hydroxylupanine Lupinus albus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.93 50000
-
 isoform A and B, gel filtration Lupinus albus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.93 tigloyl-CoA + 13-alpha-hydroxymultiflorine Lupinus albus tigloyl-CoA is (E)-2-methylcrotonoyl-CoA CoA + 13-tigloyloxymultiflorine
-
 ?
2.3.1.93 tigloyl-CoA + 13-alpha-hydroxymultiflorine Lupinus albus isoform A and B CoA + 13-tigloyloxymultiflorine
-
 ?
2.3.1.93 tigloyl-CoA + 13-hydroxylupanine Lupinus albus tigloyl-CoA is (E)-2-methylcrotonoyl-CoA CoA + 13-tigloyloxylupanine
-
 ?
2.3.1.93 tigloyl-CoA + 13-hydroxylupanine Lupinus albus isoform A and B CoA + 13-tigloyloxylupanine
-
 ?
2.3.1.93 tigloyl-CoA + 13-hydroxylupanine Lupinus albus enzyme is involved in the biosynthesis of quinolizidine alkaloid esters CoA + 13-tigloyloxylupanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.93 Cytisus scoparius
-
-
-
2.3.1.93 Lupinus albus
-
 bitter and sweet variant
-
2.3.1.93 Lupinus albus
-
 2 isoforms A and B
-
2.3.1.93 Lupinus hirsutus
-
-
-
2.3.1.93 Lupinus polyphyllus x Lupinus arboreus
-
-
-
2.3.1.93 no activity in Lupinus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.93
-
 Lupinus albus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.93 seedling
-
 Lupinus albus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.93 0.12
-
 isoform A Lupinus albus
2.3.1.93 2.18
-
 isoform B Lupinus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.93 additional information
-
 Lupinus albus ?
-
 ?
2.3.1.93 additional information substrate specificity, overview Lupinus albus ?
-
 ?
2.3.1.93 additional information baptifoline, epilupinine and lupinine are no substrates Lupinus albus ?
-
 ?
2.3.1.93 tigloyl-CoA + 13-alpha-hydroxymultiflorine tigloyl-CoA is (E)-2-methylcrotonoyl-CoA Lupinus albus CoA + 13-tigloyloxymultiflorine
-
 ?
2.3.1.93 tigloyl-CoA + 13-alpha-hydroxymultiflorine tigloyl-CoA is (E)-2-methylcrotonoyl-CoA Lupinus hirsutus CoA + 13-tigloyloxymultiflorine
-
 ?
2.3.1.93 tigloyl-CoA + 13-alpha-hydroxymultiflorine isoform A and B Lupinus albus CoA + 13-tigloyloxymultiflorine
-
 ?
2.3.1.93 tigloyl-CoA + 13-hydroxylupanine tigloyl-CoA is (E)-2-methylcrotonoyl-CoA Lupinus albus CoA + 13-tigloyloxylupanine
-
 ?
2.3.1.93 tigloyl-CoA + 13-hydroxylupanine tigloyl-CoA is (E)-2-methylcrotonoyl-CoA Cytisus scoparius CoA + 13-tigloyloxylupanine
-
 ?
2.3.1.93 tigloyl-CoA + 13-hydroxylupanine tigloyl-CoA is (E)-2-methylcrotonoyl-CoA Lupinus polyphyllus x Lupinus arboreus CoA + 13-tigloyloxylupanine
-
 ?
2.3.1.93 tigloyl-CoA + 13-hydroxylupanine isoform A and B Lupinus albus CoA + 13-tigloyloxylupanine
-
 ?
2.3.1.93 tigloyl-CoA + 13-hydroxylupanine enzyme is involved in the biosynthesis of quinolizidine alkaloid esters Lupinus albus CoA + 13-tigloyloxylupanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.93 monomer 1 * 50000, isoform A and B, SDS-PAGE Lupinus albus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.93 tigloyl:13alpha-hydroxylupanine O-tigloyltransferase HLTase Lupinus albus
2.3.1.93 tigloyl:13alpha-hydroxymultiflorine O-tigloyltransferase HMTase Lupinus albus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.93 70
-
 5 min, complete inactivation Lupinus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.93 additional information
-
 isoform 1 pI: 7.8, isoform 2 pI: 7.6 Lupinus albus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.93 0.5
-
 CoA
-
 Lupinus albus
2.3.1.93 1.7
-
 (+)-lupanine
-
 Lupinus albus
2.3.1.93 3.1
-
 (+)-epilupinine
-
 Lupinus albus