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Literature summary extracted from

  • Vogt, T.; Aebershold, R.; Ellis, B.
    Purification and characterization of sinapine synthase from seeds of Brassica napus (1993), Arch. Biochem. Biophys., 300, 622-628.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.91 choline chloride stabilizes Brassica napus
2.3.1.91 repeated freeze-thawing, stable to Brassica napus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.91 Cu2+
-
 Brassica napus
2.3.1.91 Hg2+
-
 Brassica napus
2.3.1.91 additional information no inhibition by IAA, NEM, tosylphenylalanine chloromethylketone, diethylchlorocarbamate, p-nitrophenylchloroformate Brassica napus
2.3.1.91 PMSF
-
 Brassica napus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.91 additional information no requirement for divalent cations Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.91 additional information
-
 amino acid composition and sequence Brassica napus
2.3.1.91 28000
-
 2 * 28000, SDS-PAGE Brassica napus
2.3.1.91 65000
-
 gel filtration Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.91 Brassica napus
-
 cv. Westar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.91
-
 Brassica napus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.91 seed immature dark-green Brassica napus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.91 9.6
-
 purified enzyme Brassica napus

Storage Stability

EC Number Storage Stability Organism
2.3.1.91 -70°C, at least 2 months Brassica napus
2.3.1.91 4°C, 2 weeks Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.91 1-feruloyl-beta-D-glucose + choline 40% as active as 1-sinapoylglucose Brassica napus D-glucose + feruloylcholine
-
 ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline no acceptors are betaine or CDP-choline Brassica napus D-glucose + sinapoylcholine i.e. sinapine ir
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline specific for 1-O-sinapoyl-beta-glucose Brassica napus D-glucose + sinapoylcholine i.e. sinapine ir
2.3.1.91 1-p-coumaroyl-beta-D-glucose + choline 25% Brassica napus D-glucose + p-coumaroylcholine
-
 ?
2.3.1.91 caffeoyl-beta-D-glucose + choline poor substrate Brassica napus D-glucose + caffeoylcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.91 dimer 2 * 28000, SDS-PAGE Brassica napus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.91 50
-
 15 min stable Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.91 additional information
-
 pI: 6.1 Brassica napus
2.3.1.91 6.6 7.2
-
 Brassica napus