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Literature summary extracted from

  • Vance, D.E.; Mitsuhashi, O.; Bloch, K.
    Purification and properties of the fatty acid synthetase from Mycobacterium phlei (1973), J. Biol. Chem., 248, 2303-2309.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.86 3-O-methylmannose 0.1 mM, lowers Km 4fold, activation by relieving product inhibition by binding long-chain acyl-CoA Mycolicibacterium phlei
2.3.1.86 6-O-methylglucose 0.1 mM, lowers Km 4fold, activation by relieving product inhibition by binding long-chain acyl-CoA Mycolicibacterium phlei

General Stability

EC Number General Stability Organism
2.3.1.86 low ionic strength, 0.005 M, leads to dissociation into subunits, partially reactivated by increasing the ionic strength to 0.5 M Mycolicibacterium phlei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.86 0.0096
-
 malonyl-CoA in presence of polysaccharide Mycolicibacterium phlei
2.3.1.86 0.04
-
 malonyl-CoA
-
 Mycolicibacterium phlei
2.3.1.86 0.09
-
 acetyl-CoA in presence of polysaccharide Mycolicibacterium phlei
2.3.1.86 0.8
-
 acetyl-CoA
-
 Mycolicibacterium phlei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.86 1390000
-
 sedimentation velocity data Mycolicibacterium phlei

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.86 Mycolicibacterium phlei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.86 homogeneity Mycolicibacterium phlei

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.86 low concentration of phosphate buffer causes dissociation into inactive species, reaggregation and reactivation can be partially achieved by dialysis against 0.5 M phosphate buffer Mycolicibacterium phlei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.86 0.375
-
-
 Mycolicibacterium phlei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.86 acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+ primers instead of acetyl-CoA: propionyl-CoA, butyryl-CoA or hexanoyl-CoA Mycolicibacterium phlei palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.86 NADH
-
 Mycolicibacterium phlei
2.3.1.86 NADPH requirement Mycolicibacterium phlei