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Literature summary extracted from

  • Stuible, H.P.; Meurer, G.; Schweizer, E.
    Heterologous expression and biochemical characterization of two functionally different type I fatty acid synthases from Brevibacterium ammoniagenes (1997), Eur. J. Biochem., 247, 268-273.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.85
-
 Corynebacterium ammoniagenes
2.3.1.86
-
 Corynebacterium ammoniagenes

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.85 Corynebacterium ammoniagenes
-
-
-
2.3.1.86 Corynebacterium ammoniagenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.85 purification of 2 structurally related but functionally differentiated fatty acid synthases: FAS-A and FAS-B, homogeneity Corynebacterium ammoniagenes
2.3.1.86 purification of 2 structurally related but functionally differentiated fatty acid synthases: FAS-A and FAS-B, homogeneity Corynebacterium ammoniagenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.85 0.31
-
 FAS-A Corynebacterium ammoniagenes
2.3.1.85 0.37
-
 FAS-B Corynebacterium ammoniagenes
2.3.1.86 0.31
-
 FAS-A Corynebacterium ammoniagenes
2.3.1.86 0.37
-
 FAS-B Corynebacterium ammoniagenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
-
 Corynebacterium ammoniagenes palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O FAS-A mainly synthesizes the C18 fatty acids oleate and stearate with only traces of palmitate, the major product of FAS-B is pamitate ?
2.3.1.86 acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+
-
 Corynebacterium ammoniagenes palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O FAS-A mainly synthesizes the C18 fatty acids oleate and stearate with only traces of palmitate, the major product of FAS-B is pamitate ?

Subunits

EC Number Subunits Comment Organism
2.3.1.85 More identical subunits, SDS-PAGE Corynebacterium ammoniagenes
2.3.1.86 More identical subunits, SDS-PAGE Corynebacterium ammoniagenes