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Literature summary extracted from

  • Minetoki, T.; Bogaki, T.; Iwamatsu, A.; Fujii, T.; Hamachi, M.
    The purification, properties and internal peptide sequences of alcohol acetyltransferase isolated from Saccharomyces cerevisiae Kyokai No. 7 (1993), Biosci. Biotechnol. Biochem., 57, 2094-2098.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.84 5,5'-dithiobis(2-nitrobenzoate) complete inhibition Saccharomyces cerevisiae
2.3.1.84 Cd2+
-
 Saccharomyces cerevisiae
2.3.1.84 Cu2+
-
 Saccharomyces cerevisiae
2.3.1.84 Hg2+
-
 Saccharomyces cerevisiae
2.3.1.84 linoleic acid 2 mM, 86% inhibition Saccharomyces cerevisiae
2.3.1.84 linolenic acid 2 mM, 68% inhibition Saccharomyces cerevisiae
2.3.1.84 p-chloromercuribenzoate complete inhibition Saccharomyces cerevisiae
2.3.1.84 Zn2+
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.84 0.025
-
 acetyl-CoA strain NCYC 366 Saccharomyces cerevisiae
2.3.1.84 0.19
-
 acetyl-CoA
-
 Saccharomyces cerevisiae
2.3.1.84 29.8
-
 isoamyl alcohol
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.84 22000
-
 x * 22000, SDS-PAGE Cladosporium cladosporioides
2.3.1.84 60000
-
 x * 60000, SDS-PAGE Saccharomyces cerevisiae
2.3.1.84 270000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.84 Cladosporium cladosporioides
-
-
-
2.3.1.84 Saccharomyces cerevisiae
-
 sake yeast strain Kyokai No. 7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.84 PBE 94 column, DEAE-cellulose, Toypearl HW60, hydroxyapatite, octyl Sepharose Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.84 5.47
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.84 isoamylalcohol + acetyl-CoA slightly higher activity with straight-chain alcohols than with branched-chain alcohols, activity with straight-chain alcohols in descending order: pentanol, butanol, propanol, very low activity with ethanol, activity with branched-chain alcohols in descending order of carbon number: C5, C4, C3 Saccharomyces cerevisiae isoamyl acetate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.84 ? x * 60000, SDS-PAGE Saccharomyces cerevisiae
2.3.1.84 ? x * 22000, SDS-PAGE Cladosporium cladosporioides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.84 25
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.84 10
-
 loss of 20% activity Saccharomyces cerevisiae
2.3.1.84 20
-
 rapid inactivation above Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.84 5.9
-
-
 Cladosporium cladosporioides
2.3.1.84 8
-
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.84 7.5 8 no loss of activity after 22 h at 4°C Saccharomyces cerevisiae