Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rasche, M.E.; Smith, K.S.; Ferry, J.G.
    Identification of cysteine and arginine residues essential for the phosphotransacetylase from Methanosarcina thermophila (1997), J. Bacteriol., 179, 7712-7717.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.8
-
 Methanosarcina thermophila

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.8 C159A Km similar to wild-type enzyme Methanosarcina thermophila
2.3.1.8 C159A/C277A/C312A/C325A Km similar to wild-type enzyme Methanosarcina thermophila
2.3.1.8 C159S Km similar to wild-type enzyme Methanosarcina thermophila
2.3.1.8 C277A Km similar to wild-type enzyme Methanosarcina thermophila
2.3.1.8 C277A/C312A/C325A Km similar to wild-type enzyme Methanosarcina thermophila
2.3.1.8 C312A Km similar to wild-type enzyme Methanosarcina thermophila
2.3.1.8 C325A Km similar to wild-type enzyme Methanosarcina thermophila
2.3.1.8 R133Q increased Km for CoA, decreased Km for acetyl phosphate Methanosarcina thermophila
2.3.1.8 R287Q decreased Km for CoA Methanosarcina thermophila
2.3.1.8 R28Q increased Km Methanosarcina thermophila
2.3.1.8 R310Q decreased Km for CoA Methanosarcina thermophila
2.3.1.8 R87Q increased Km for CoA, decreased Km for acetyl phosphate Methanosarcina thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 N-ethylmaleimide
-
 Methanosarcina thermophila
2.3.1.8 Phenylglyoxal 33% inhibition after preincubation with phenylglyoxal Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.8 0.034
-
 CoA R287Q mutant Methanosarcina thermophila
2.3.1.8 0.037
-
 CoA R310Q mutant Methanosarcina thermophila
2.3.1.8 0.043
-
 acetyl phosphate R87Q mutant Methanosarcina thermophila
2.3.1.8 0.071
-
 CoA C159S mutant Methanosarcina thermophila
2.3.1.8 0.073
-
 acetyl phosphate R133Q mutant Methanosarcina thermophila
2.3.1.8 0.073
-
 CoA C159A/C277A/C312A/C325A mutant Methanosarcina thermophila
2.3.1.8 0.078
-
 CoA C325A mutant Methanosarcina thermophila
2.3.1.8 0.086
-
 CoA C277A/C312A/C325A mutant Methanosarcina thermophila
2.3.1.8 0.089
-
 CoA wild-type after expression in E. coli Methanosarcina thermophila
2.3.1.8 0.09
-
 CoA wild tpye Methanosarcina thermophila
2.3.1.8 0.092
-
 CoA C312A mutant Methanosarcina thermophila
2.3.1.8 0.093
-
 CoA C159A mutant Methanosarcina thermophila
2.3.1.8 0.093
-
 CoA C277A mutant Methanosarcina thermophila
2.3.1.8 0.166
-
 acetyl phosphate R287Q mutant Methanosarcina thermophila
2.3.1.8 0.17
-
 acetyl phosphate wild tpye Methanosarcina thermophila
2.3.1.8 0.18
-
 acetyl phosphate wild-type after expression in E. coli Methanosarcina thermophila
2.3.1.8 0.187
-
 acetyl phosphate C277A mutant Methanosarcina thermophila
2.3.1.8 0.191
-
 acetyl phosphate C159S mutant Methanosarcina thermophila
2.3.1.8 0.198
-
 acetyl phosphate C312A mutant Methanosarcina thermophila
2.3.1.8 0.206
-
 acetyl phosphate C159A mutant Methanosarcina thermophila
2.3.1.8 0.222
-
 acetyl phosphate C159A/C277A/C312A/C325A mutant Methanosarcina thermophila
2.3.1.8 0.23
-
 acetyl phosphate C325A mutant Methanosarcina thermophila
2.3.1.8 0.253
-
 CoA R28Q mutant Methanosarcina thermophila
2.3.1.8 0.254
-
 acetyl phosphate C277A/C312A/C325A mutant Methanosarcina thermophila
2.3.1.8 0.747
-
 CoA R87Q mutant Methanosarcina thermophila
2.3.1.8 0.775
-
 acetyl phosphate R310Q mutant Methanosarcina thermophila
2.3.1.8 1.32
-
 acetyl phosphate R28Q mutant Methanosarcina thermophila
2.3.1.8 1.694
-
 CoA R133Q mutant Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Methanosarcina thermophila
-
 CoA + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Methanosarcina thermophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8
-
 Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Methanosarcina thermophila CoA + acetyl phosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.8 1.32
-
 acetyl phosphate C159A mutant Methanosarcina thermophila
2.3.1.8 1.42
-
 CoA R310Q mutant Methanosarcina thermophila
2.3.1.8 1.48
-
 CoA C159A mutant Methanosarcina thermophila
2.3.1.8 1.63
-
 acetyl phosphate R310Q mutant Methanosarcina thermophila
2.3.1.8 3.03
-
 acetyl phosphate C159A/C277A/C312A/C325A mutant Methanosarcina thermophila
2.3.1.8 3.3
-
 CoA C159A/C277A/C312A/C325A mutant Methanosarcina thermophila
2.3.1.8 4.93
-
 acetyl phosphate R87Q mutant Methanosarcina thermophila
2.3.1.8 5.45
-
 acetyl phosphate mutant R287Q Methanosarcina thermophila
2.3.1.8 5.95
-
 CoA mutant R287Q Methanosarcina thermophila
2.3.1.8 12.5
-
 acetyl phosphate R133Q mutant Methanosarcina thermophila
2.3.1.8 24.1
-
 acetyl phosphate mutant R28Q Methanosarcina thermophila
2.3.1.8 24.6
-
 CoA R87Q mutant Methanosarcina thermophila
2.3.1.8 26
-
 CoA mutant R28Q Methanosarcina thermophila
2.3.1.8 40.6
-
 acetyl phosphate wild type Methanosarcina thermophila
2.3.1.8 44.5
-
 acetyl phosphate C312A mutant Methanosarcina thermophila
2.3.1.8 45.6
-
 CoA R133Q mutant Methanosarcina thermophila
2.3.1.8 51.6
-
 acetyl phosphate C277A/C312A/C325A mutant Methanosarcina thermophila
2.3.1.8 53.6
-
 CoA C277A/C312A/C325A mutant Methanosarcina thermophila
2.3.1.8 53.8
-
 CoA wild type Methanosarcina thermophila
2.3.1.8 55.4
-
 CoA C312A mutant Methanosarcina thermophila
2.3.1.8 57.6
-
 acetyl phosphate C325A mutant Methanosarcina thermophila
2.3.1.8 59.9
-
 CoA C325A mutant Methanosarcina thermophila
2.3.1.8 66.3
-
 acetyl phosphate C159S mutant Methanosarcina thermophila
2.3.1.8 68.5
-
 acetyl phosphate wild-type after expression in E. coli Methanosarcina thermophila
2.3.1.8 73.5
-
 CoA C159S mutant Methanosarcina thermophila
2.3.1.8 87.5
-
 CoA wild-type after expression in E. coli Methanosarcina thermophila
2.3.1.8 94.9
-
 acetyl phosphate C277A mutant Methanosarcina thermophila
2.3.1.8 100
-
 CoA C277A mutant Methanosarcina thermophila