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Literature summary extracted from

  • Iyer, P.P.; Ferry, J.G.
    Role of arginines in coenzyme A binding and catalysis by the phosphotransacetylase from Methanosarcina thermophila (2001), J. Bacteriol., 183, 4244-4250.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.8 R133A altered kinetic properties, increased Km for CoA Methanosarcina thermophila
2.3.1.8 R133E altered kinetic properties, increased Km for CoA Methanosarcina thermophila
2.3.1.8 R133K altered kinetic properties, increased Km for CoA Methanosarcina thermophila
2.3.1.8 R133Q altered kinetic properties, increased Km for CoA Methanosarcina thermophila
2.3.1.8 R87A altered kinetic properties, increased Km for CoA Methanosarcina thermophila
2.3.1.8 R87E altered kinetic properties, increased Km for CoA Methanosarcina thermophila
2.3.1.8 R87K altered kinetic properties, increased Km for CoA Methanosarcina thermophila
2.3.1.8 R87Q altered kinetic properties, increased Km for CoA Methanosarcina thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 2,3-Butanedione almost complete loss of wild-type enzyme activity after 10 min at 10 mM Methanosarcina thermophila
2.3.1.8 desulfo-CoA strong competitive inhibitor Methanosarcina thermophila
2.3.1.8 phosphate
-
 Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.8 0.07
-
 CoA wild-type Methanosarcina thermophila
2.3.1.8 0.11
-
 CoA R133K mutant Methanosarcina thermophila
2.3.1.8 0.27
-
 CoA R87E mutant Methanosarcina thermophila
2.3.1.8 0.36
-
 CoA R133A mutant Methanosarcina thermophila
2.3.1.8 0.7
-
 CoA R133Q mutant Methanosarcina thermophila
2.3.1.8 0.7
-
 CoA R133E mutant Methanosarcina thermophila
2.3.1.8 0.75
-
 CoA R87K mutant Methanosarcina thermophila
2.3.1.8 0.8
-
 desulfo-CoA R87E mutant Methanosarcina thermophila
2.3.1.8 0.8
-
 desulfo-CoA R133K mutant Methanosarcina thermophila
2.3.1.8 0.9
-
 CoA R87A mutant Methanosarcina thermophila
2.3.1.8 1
-
 desulfo-CoA R133E mutant Methanosarcina thermophila
2.3.1.8 1.1
-
 CoA R87Q mutant Methanosarcina thermophila
2.3.1.8 1.3
-
 desulfo-CoA R87Q mutant Methanosarcina thermophila
2.3.1.8 1.3
-
 desulfo-CoA R133A mutant Methanosarcina thermophila
2.3.1.8 1.4
-
 desulfo-CoA wild-type Methanosarcina thermophila
2.3.1.8 3.9
-
 desulfo-CoA R87K mutant Methanosarcina thermophila
2.3.1.8 4
-
 desulfo-CoA R133Q mutant Methanosarcina thermophila
2.3.1.8 6
-
 desulfo-CoA R87A mutant Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Methanosarcina thermophila
-
 CoA + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Methanosarcina thermophila
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Methanosarcina thermophila CoA + acetyl phosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.8 0.00467
-
 desulfo-CoA R133E mutant Methanosarcina thermophila
2.3.1.8 0.0125
-
 desulfo-CoA R133A mutant Methanosarcina thermophila
2.3.1.8 0.133
-
 desulfo-CoA R133K mutant Methanosarcina thermophila
2.3.1.8 0.283
-
 CoA R87E mutant Methanosarcina thermophila
2.3.1.8 0.383
-
 desulfo-CoA R87E mutant Methanosarcina thermophila
2.3.1.8 0.467
-
 desulfo-CoA R133Q mutant Methanosarcina thermophila
2.3.1.8 0.683
-
 desulfo-CoA R87K mutant Methanosarcina thermophila
2.3.1.8 0.733
-
 CoA R133E mutant Methanosarcina thermophila
2.3.1.8 1.63
-
 desulfo-CoA R87Q mutant Methanosarcina thermophila
2.3.1.8 3.07
-
 desulfo-CoA R87A mutant Methanosarcina thermophila
2.3.1.8 4.53
-
 desulfo-CoA wild-type Methanosarcina thermophila
2.3.1.8 6.22
-
 CoA R87K mutant Methanosarcina thermophila
2.3.1.8 6.97
-
 CoA R133K mutant Methanosarcina thermophila
2.3.1.8 10.5
-
 CoA R133A mutant Methanosarcina thermophila
2.3.1.8 19.1
-
 CoA R133Q mutant Methanosarcina thermophila
2.3.1.8 20.4
-
 CoA R87A mutant Methanosarcina thermophila
2.3.1.8 23.2
-
 CoA R87Q mutant Methanosarcina thermophila
2.3.1.8 81.3
-
 CoA wild-type Methanosarcina thermophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.8 0.001
-
 desulfo-CoA
-
 Methanosarcina thermophila
2.3.1.8 0.32
-
 phosphate R133Q mutant Methanosarcina thermophila
2.3.1.8 0.52
-
 phosphate R87Q mutant Methanosarcina thermophila
2.3.1.8 0.81
-
 phosphate wild-type enzyme Methanosarcina thermophila