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Literature summary extracted from

  • MacDonald, P.N.; Ong, D.E.
    Assay of lecithin-retinol acyltransferase (1990), Methods Enzymol., 189, 450-459.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.76 medicine retinyl esterification: storage of vitamin A Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.76 cellular retinol-binding protein specific inhibition of retinol fatty-acyltransferase by capturing substrate Rattus norvegicus
2.3.1.76 additional information not inhibited by PMSF Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.76 microsome membrane fraction Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.76 acyl-CoA + retinol Rattus norvegicus
-
 CoA + retinyl ester
-
 ?
2.3.1.76 acyl-CoA + retinol Rattus norvegicus Sprague-Dawley
-
 CoA + retinyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.76 Rattus norvegicus
-
 Sprague-Dawley
-
2.3.1.76 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.76 partial purification by differential centrifugation Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.76 small intestine
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.76 additional information
-
 activity determined from microsomes Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.76 -70ºC, phosphate buffer, pH 7.2, DTT, 12 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.76 acyl-CoA + retinol
-
 Rattus norvegicus CoA + retinyl ester
-
 ?
2.3.1.76 acyl-CoA + retinol
-
 Rattus norvegicus Sprague-Dawley CoA + retinyl ester
-
 ?
2.3.1.76 palmitoyl-CoA + retinol
-
 Rattus norvegicus CoA + retinylpalmitate
-
 ?
2.3.1.76 palmitoyl-CoA + retinol
-
 Rattus norvegicus Sprague-Dawley CoA + retinylpalmitate
-
 ?