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Literature summary extracted from

  • Tropf, S.; Kaercher, B.; Schroeder, G.; Schroeder, J.
    Reaction mechanisms of homodimeric plant polyketide synthases (stilbene and chalcone synthase). A single active site for the condensing reaction is sufficient for synthesis of stilbenes, chalcones, and 6'-deoxychalcones (1995), J. Biol. Chem., 270, 7922-7928.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74
-
Sinapis alba

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.74 C169S no enzyme activity Sinapis alba
2.3.1.74 C169S/Q100E 28% of wild-type activity Sinapis alba
2.3.1.74 C169S/Q100E/K180Q 15% of wild-type activity Sinapis alba
2.3.1.74 K180Q no enzyme activity Sinapis alba
2.3.1.74 Q100E 14% of wild-type activity Sinapis alba
2.3.1.74 S158C 90% of wild-type activity Sinapis alba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.0023
-
4-coumaroyl-CoA C169S/Q100E/K180Q triple mutant Sinapis alba
2.3.1.74 0.0025
-
4-coumaroyl-CoA wild-type enzyme Sinapis alba

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Sinapis alba
-
white mustard
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 malonyl CoA + 4-coumaroyl-CoA + NADH
-
Sinapis alba 6'-deoxychalcone + CoA + CO2 + NAD+ the formation of this product requires an additional reductase ?