EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.74 | - |
Sinapis alba |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.74 | C169S | no enzyme activity | Sinapis alba |
2.3.1.74 | C169S/Q100E | 28% of wild-type activity | Sinapis alba |
2.3.1.74 | C169S/Q100E/K180Q | 15% of wild-type activity | Sinapis alba |
2.3.1.74 | K180Q | no enzyme activity | Sinapis alba |
2.3.1.74 | Q100E | 14% of wild-type activity | Sinapis alba |
2.3.1.74 | S158C | 90% of wild-type activity | Sinapis alba |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.74 | 0.0023 | - |
4-coumaroyl-CoA | C169S/Q100E/K180Q triple mutant | Sinapis alba | |
2.3.1.74 | 0.0025 | - |
4-coumaroyl-CoA | wild-type enzyme | Sinapis alba |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.74 | Sinapis alba | - |
white mustard | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.74 | malonyl CoA + 4-coumaroyl-CoA + NADH | - |
Sinapis alba | 6'-deoxychalcone + CoA + CO2 + NAD+ | the formation of this product requires an additional reductase | ? |