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Literature summary extracted from

  • Morita, H.; Takahashi, Y.; Noguchi, H.; Abe, I.
    Enzymatic Formation of Unnatural Aromatic Polyketides by Chalcone Synthase (2000), Biochem. Biophys. Res. Commun., 279, 190-195.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74
-
 Scutellaria baicalensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.0361
-
 4-coumaroyl-CoA
-
 Scutellaria baicalensis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Scutellaria baicalensis
-
 labiatae
-
2.3.1.220 Scutellaria baicalensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74
-
 Scutellaria baicalensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 leaf
-
 Scutellaria baicalensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA
-
 Scutellaria baicalensis naringenin chalcone + 4 CoA + 3 CO2
-
 ?
2.3.1.74 malonyl-CoA + benzoyl-CoA
-
 Scutellaria baicalensis phlorobenzophenone + CO2 + CoA i.e. 2,4,6-trihydroxybenzophenone along with byproducts ?
2.3.1.74 malonyl-CoA + phenylacetyl-CoA
-
 Scutellaria baicalensis phlorobenzylketone + CoA + Co2 i.e. 2,4,6-trihydroxyphenylbenzylketone ?
2.3.1.74 additional information enzyme accepts both aromatic and aliphatic CoA esters as starter substrate Scutellaria baicalensis ?
-
 ?
2.3.1.220 3 malonyl-CoA + benzoyl-CoA
-
 Scutellaria baicalensis 4 CoA + 2,4,6-trihydroxybenzophenone + 3 CO2
-
 ?
2.3.1.220 3 malonyl-CoA + hexanoyl-CoA
-
 Scutellaria baicalensis 4 CoA + 4-hydroxy-6-pentyl-2H-pyran-2-one + 4-hydroxy-6-(2-oxoheptyl)-2H-pyran-2-one + 3 CO2
-
 ?
2.3.1.220 3 malonyl-CoA + isobutyryl-CoA
-
 Scutellaria baicalensis 4 CoA + phloroisobutyrophenone + 3 CO2
-
 ?
2.3.1.220 3 malonyl-CoA + isovaleryl-CoA
-
 Scutellaria baicalensis 4 CoA + phloroisovalerophenone + 3 CO2
-
 ?
2.3.1.220 3 malonyl-CoA + phenylacetyl-CoA
-
 Scutellaria baicalensis 4 CoA + 2,4,6-trihydroxyphenylbenzylketone + 3 CO2
-
 ?
2.3.1.220 additional information chalcone synthase, CHS EC 2.3.1.74, shows broad substrate specificity, overview. Both aromatic and aliphatic CoA esters are accepted in the active site of the enzyme as a starter substrate for the complex condensation reaction. CHS converts benzoyl-CoA to phlorobenzophenone, i.e. 2,4,6-trihydroxybenzophenone, along with pyrone by-products, and it converts phenylacetyl-CoA to an unnatural aromatic polyketide, phlorobenzylketone, i.e. 2,4,6-trihydroxyphenylbenzylketone. Scutellaria baicalensis CHS also accepts aliphatic CoA esters, isovaleryl-CoA and isobutyryl-CoA, to produce phloroacylphenones. Hexanoyl-CoA only affords pyrone derivatives without formation of a new aromatic ring Scutellaria baicalensis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.220 30
-
 assay at Scutellaria baicalensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.74 0.021
-
 4-coumaroyl-CoA
-
 Scutellaria baicalensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.220 7.5
-
 assay at Scutellaria baicalensis