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Literature summary extracted from

  • Beerhues, L.; Wiermann, R.
    Chalcone synthases from spinach (Spinacia oleracea L.). I. Purification, peptide patterns, and immunological properties of different forms (1988), Planta, 173, 532-543.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.74 5% activity after freezing for 1 h without presence of glycerol Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.0008
-
 4-coumaroyl-CoA isozyme AI Spinacia oleracea
2.3.1.74 0.001
-
 4-coumaroyl-CoA isozyme AII Spinacia oleracea
2.3.1.74 0.0019
-
 malonyl-CoA isozyme AII Spinacia oleracea
2.3.1.74 0.002
-
 malonyl-CoA isozyme AI Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.74 additional information no activity in chloroplasts Spinacia oleracea
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.74 48000
-
 isozyme AI, gel filtration, differences in MW may be due to different spatial conformations of AI and AII Spinacia oleracea
2.3.1.74 62000
-
 isozyme AII, gel filtration, differences in MW may be due to different spatial conformations of AI and AII Spinacia oleracea
2.3.1.74 78000
-
 isozyme AII, non-denaturing gel electrophoresis, differences in MW may be due to different spatial conformations of AI and AII Spinacia oleracea
2.3.1.74 88000
-
 isozyme AI, non-denaturing gel electrophoresis, differences in MW may be due to different spatial conformations of AI and AII Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 leaf
-
 Spinacia oleracea
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.74 -20°C, 0.4 M phosphate buffer, pH 6.5, 20% v/v glycerol, 4 weeks Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 malonyl-CoA + 4-coumaroyl-CoA
-
 Spinacia oleracea naringenin chalcone + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.74 dimer 1 * 78000 + 1 * 88000, non-denaturating PAGE, 1 * 48000 + 1 * 62000, gel filtration Spinacia oleracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.74 4
-
 half-life 4-5 d, partially purified enzyme Spinacia oleracea