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Literature summary extracted from

  • Lanz, T.; Tropf, S.; Marner, F.J.; Schröder, J.; Schröder, G.
    The role of cysteines in polyketide synthases. Site-directed mutagenesis of resveratrol and chalcone synthases, two key enzymes in different plant-specific pathways (1991), J. Biol. Chem., 266, 9971-9976.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74 expression in Escherichia coli, site directed mutagenesis, sequence alignment with EC 2.3.1.95 Sinapis alba
2.3.1.95 sequence alignment with naringenin-chalcone synthase, EC 2.3.1.74 Arachis hypogaea

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.74 C135A 77% enzyme activity Sinapis alba
2.3.1.74 C169A no enzyme activity Sinapis alba
2.3.1.74 C195A increased enzyme activity Sinapis alba
2.3.1.74 C347A 44% enzyme activity Sinapis alba
2.3.1.74 C65A 15% enzyme activity Sinapis alba
2.3.1.74 C89A 71% enzyme activity Sinapis alba

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.74 cerulenin 50% inhibition at 0.001 mg per assay Sinapis alba

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Sinapis alba
-
-
-
2.3.1.95 Arachis hypogaea
-
peanut
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 malonyl-CoA + 4-coumaroyl-CoA
-
Sinapis alba naringenin chalcone + CoA + CO2 due to the presence of isomerase the immediate product naringenin chalcone is not detectable, instead 4,2',4',6'-tetrahydroxychalcone is found, the latter cyclizes spontaneously to naringenin ?