EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.74 | expression in Escherichia coli, site directed mutagenesis, sequence alignment with EC 2.3.1.95 | Sinapis alba |
2.3.1.95 | sequence alignment with naringenin-chalcone synthase, EC 2.3.1.74 | Arachis hypogaea |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.74 | C135A | 77% enzyme activity | Sinapis alba |
2.3.1.74 | C169A | no enzyme activity | Sinapis alba |
2.3.1.74 | C195A | increased enzyme activity | Sinapis alba |
2.3.1.74 | C347A | 44% enzyme activity | Sinapis alba |
2.3.1.74 | C65A | 15% enzyme activity | Sinapis alba |
2.3.1.74 | C89A | 71% enzyme activity | Sinapis alba |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.74 | cerulenin | 50% inhibition at 0.001 mg per assay | Sinapis alba |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.74 | Sinapis alba | - |
- |
- |
2.3.1.95 | Arachis hypogaea | - |
peanut | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.74 | malonyl-CoA + 4-coumaroyl-CoA | - |
Sinapis alba | naringenin chalcone + CoA + CO2 | due to the presence of isomerase the immediate product naringenin chalcone is not detectable, instead 4,2',4',6'-tetrahydroxychalcone is found, the latter cyclizes spontaneously to naringenin | ? |