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Literature summary extracted from

  • Kesy, J.M.; Bandurski, R.S.
    Partial purification and characterization of indol-3-ylacetylglucoe:myo-inositol indol-3-ylacetyltransferase (indoleacetic acid-inositol synthase) (1990), Plant Physiol., 94, 1598-1604.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.3.1.72 lyophilization, stable to Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.72 2-mercaptoethanol 74% inhibition at 5 mM Zea mays
2.3.1.72 Dithionite inhibition at 40 mM Zea mays
2.3.1.72 dithiothreitol 98% inhibition at 5 mM Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.72 0.03
-
 1-O-indol-3-yl-acetyl-beta-D-glucose
-
 Zea mays
2.3.1.72 4.1
-
 myo-inositol
-
 Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.72 59000
-
 gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.72 indole-3-acetyl-beta-1-D-glucoside + myo-inositol Zea mays involved in biosynthesis of indole-3-acetyl-beta-1,4-glucan D-glucose + indole-3-acetyl-myo-inositol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.72 Zea mays
-
 sweet corn, var. Seneca Horizon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.72
-
 Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.72 kernel endosperm Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.72 13.3
-
-
 Zea mays

Storage Stability

EC Number Storage Stability Organism
2.3.1.72 -20°C, crude liquid endosperm, several years, no loss of activity Zea mays
2.3.1.72 4°C, more than 2 months Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.72 indole-3-acetyl-beta-1-D-glucoside + myo-inositol scyllo-inositol or myo-inosose-2 can replace myo-inositol Zea mays D-glucose + indole-3-acetyl-myo-inositol
-
 ?
2.3.1.72 indole-3-acetyl-beta-1-D-glucoside + myo-inositol myo-inositol-D-galactopyranose, cyclohexanol, mannitol, glycerol Zea mays D-glucose + indole-3-acetyl-myo-inositol
-
 ?
2.3.1.72 indole-3-acetyl-beta-1-D-glucoside + myo-inositol involved in biosynthesis of indole-3-acetyl-beta-1,4-glucan Zea mays D-glucose + indole-3-acetyl-myo-inositol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.72 25
-
 assay at Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.72 additional information
-
 pI: 6.1 Zea mays