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Literature summary extracted from

  • Umezawa, H.
    Biosynthesis of leupeptin. III. Isolation and properties of an enzyme synthesizing acetyl-L-leucine (1980), J. Antibiot., 33, 857-862.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.66 Mg2+ weak Streptomyces roseus
2.3.1.66 Mn2+ weak Streptomyces roseus
2.3.1.66 N-ethylmaleimide strong Streptomyces roseus
2.3.1.66 p-chloromercuribenzoate strong Streptomyces roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.66 0.005
-
 acetyl-CoA
-
 Streptomyces roseus
2.3.1.66 0.06
-
 L-leucine
-
 Streptomyces roseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.66 Cu2+ slight stimulation Streptomyces roseus
2.3.1.66 Fe2+ stimulates strongly Streptomyces roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.66 27000
-
 gel filtration Streptomyces roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.66 acetyl-CoA + L-leucine Streptomyces roseus
-
 CoA + N-acetyl-L-leucine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.66 Streptomyces roseus
-
 strain MA839-A1, also present in strain MA839-A1 LN-S
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.66 partial Streptomyces roseus

Storage Stability

EC Number Storage Stability Organism
2.3.1.66 -180°C, 3 months, 70% remaining activity Streptomyces roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.66 acetyl-CoA + L-arginine
-
 Streptomyces roseus CoA + ?
-
 ?
2.3.1.66 acetyl-CoA + L-leucine
-
 Streptomyces roseus CoA + N-acetyl-L-leucine
-
 ?
2.3.1.66 acetyl-CoA + L-phenylalanine
-
 Streptomyces roseus CoA + N-acetyl-L-phenylalanine
-
 ?
2.3.1.66 acetyl-CoA + L-valine
-
 Streptomyces roseus CoA + N-acetyl-L-valine
-
 ?
2.3.1.66 additional information dipeptides, e.g. L-leucyl-L-leucine, are acetylated more rapidly than L-leucine or peptides with more than two amino acids, D-isomers are acetylated more slowly than L-isomers Streptomyces roseus ?
-
 ?
2.3.1.66 propionyl-CoA + L-leucine 10% of activity compared to acetyl-CoA Streptomyces roseus CoA + propionyl-L-leucine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.66 27
-
 assay at Streptomyces roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.66 9
-
 assay at Streptomyces roseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.66 7 9 10% of maximal activity at pH 7, maximal activity at pH 9 Streptomyces roseus