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Literature summary extracted from

  • Vessey, D.A.
    The co-purification and common identity of cholyl CoA:glycine- and cholyl CoA: taurine-N-acyltransferase activities from bovine liver (1979), J. Biol. Chem., 254, 2059-2063.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.65 freezing inactivates Bos taurus
2.3.1.65 high ionic strength stabilizes Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.65 additional information
-
 additional information
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.65 soluble
-
 Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.65 47000
-
 gel filtration Bos taurus
2.3.1.65 50900
-
 1 * 50900, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.65 choloyl-CoA + glycine Bos taurus conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine Bos taurus conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct CoA + taurocholate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.65 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.65 900fold Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.65 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.65 35.2
-
 taurine Bos taurus
2.3.1.65 46.9
-
 glycine Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.3.1.65 0°C, 50 mM phosphate buffer, pH 7.8, with 0.5 M NaCl, 2 mM dithioerythritol, 1 month, 25% loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.65 choloyl-CoA + glycine
-
 Bos taurus CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + glycine conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct Bos taurus CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine
-
 Bos taurus CoA + taurocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct Bos taurus CoA + taurocholate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.65 monomer 1 * 50900, SDS-PAGE Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.65 additional information
-
 taurocholate stabilizes against temperature denaturation Bos taurus
2.3.1.65 50
-
 10 min, stable up to Bos taurus
2.3.1.65 57
-
 10 min, about 70% loss of activity Bos taurus
2.3.1.65 60
-
 inactivation within 10 min Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.65 additional information
-
 additional information higher kcat for glycine than for taurine Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.65 additional information
-
 pI: 6.6 Bos taurus