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Literature summary extracted from

  • Polsky, R.; Shuster, L.
    Preparation and characterization of two isozymes of choline acetyltransferase from squid head ganglia. I. Purification and properties (1976), Biochim. Biophys. Acta, 445, 25-42.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.6 NaCl
-
 Doryteuthis pealeii
2.3.1.6 NaCl
-
 Doryteuthis opalescens

General Stability

EC Number General Stability Organism
2.3.1.6 bovine serum albumin stabilizes Doryteuthis pealeii
2.3.1.6 bovine serum albumin stabilizes Doryteuthis opalescens
2.3.1.6 EDTA stabilizes Doryteuthis pealeii
2.3.1.6 EDTA stabilizes Doryteuthis opalescens
2.3.1.6 sucrose stabilizes Doryteuthis pealeii
2.3.1.6 sucrose stabilizes Doryteuthis opalescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.6 5,5'-dithiobis(2-nitrobenzoate)
-
 Doryteuthis opalescens
2.3.1.6 5,5'-dithiobis(2-nitrobenzoate)
-
 Doryteuthis pealeii
2.3.1.6 N-ethylmaleimide
-
 Doryteuthis opalescens
2.3.1.6 N-ethylmaleimide
-
 Doryteuthis pealeii
2.3.1.6 Phenylmethanesulfonylfluoride
-
 Doryteuthis opalescens
2.3.1.6 Phenylmethanesulfonylfluoride
-
 Doryteuthis pealeii
2.3.1.6 Reactive blue 2
-
 Doryteuthis opalescens
2.3.1.6 Reactive blue 2
-
 Doryteuthis pealeii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.6 0.0082 0.032 acetyl-CoA
-
 Doryteuthis pealeii
2.3.1.6 4.4 19 choline
-
 Doryteuthis pealeii

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.6 Doryteuthis opalescens
-
 squid
-
2.3.1.6 Doryteuthis pealeii
-
 squid
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.6 2 isozymes Doryteuthis pealeii
2.3.1.6 2 isozymes Doryteuthis opalescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.6 ganglion head Doryteuthis pealeii
-
2.3.1.6 ganglion head Doryteuthis opalescens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.6 additional information
-
-
 Doryteuthis pealeii
2.3.1.6 additional information
-
-
 Doryteuthis opalescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.6 acetyl-CoA + choline
-
 Doryteuthis pealeii acetylcholine + CoA
-
 ?
2.3.1.6 acetyl-CoA + choline
-
 Doryteuthis opalescens acetylcholine + CoA
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.6 37
-
 60% sucrose, 1 mg/ml bovine serum albumin, 2 h stable Doryteuthis pealeii
2.3.1.6 37
-
 60% sucrose, 1 mg/ml bovine serum albumin, 2 h stable Doryteuthis opalescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.6 additional information
-
 broad optimum in alkaline range Doryteuthis pealeii
2.3.1.6 additional information
-
 broad optimum in alkaline range Doryteuthis opalescens