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Literature summary extracted from

  • Della Ragione, F.; Pegg, A.E.
    Purification and characterization of spermidine/spermine N1-acetyltransferase from rat liver (1982), Biochemistry, 21, 6152-6158.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.57 additional information enzyme is induced by treatment with hepatotoxins, e.g. carbon tetrachloride Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.57 unstable enzyme, spermidine stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.57 3'-dephospho-CoA less inhibition than by CoA Rattus norvegicus
2.3.1.57 CoA strong inhibitor Rattus norvegicus
2.3.1.57 CoA methyl disulfide very strong inhibitor Rattus norvegicus
2.3.1.57 p-chloromercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.57 0.0015
-
 acetyl-CoA
-
 Rattus norvegicus
2.3.1.57 0.008
-
 sym-norspermidine
-
 Rattus norvegicus
2.3.1.57 0.03
-
 N1-acetylspermine
-
 Rattus norvegicus
2.3.1.57 0.035
-
 spermine
-
 Rattus norvegicus
2.3.1.57 0.1
-
 1,5-Diaminopentane
-
 Rattus norvegicus
2.3.1.57 0.13
-
 spermidine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.57 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.57 60000
-
 2 * 60000, SDS-PAGE Rattus norvegicus
2.3.1.57 115000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.57 acetyl-CoA + spermidine Rattus norvegicus spermidine and spermine may be physiological substrates, enzyme may play an important role in interconversion of polyamines CoA + N1-acetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermine Rattus norvegicus spermidine and spermine may be physiological substrates, enzyme may play an important role in interconversion of polyamines CoA + N1-acetylspermine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.57 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.57 112000fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.57 liver carbon tetrachloride induced liver Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.57 8.102
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.57 -20°C, 50 mM Tris-HCl buffer, pH 7.5, 10 mM spermidine, per week, 30% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.57 acetyl-CoA + 1,5-diaminopentane
-
 Rattus norvegicus CoA + N1-acetyl-1,5-diaminopentane
-
 ?
2.3.1.57 acetyl-CoA + N1-acetylspermine reaction in vitro, but not in vivo Rattus norvegicus CoA + N1,N12-diacetylspermine
-
 ir
2.3.1.57 acetyl-CoA + spermidine physiological substrate, higher rate than with spermine Rattus norvegicus CoA + N1-acetylspermidine product is exclusively N1-acetylspermidine ir
2.3.1.57 acetyl-CoA + spermidine spermidine and spermine may be physiological substrates, enzyme may play an important role in interconversion of polyamines Rattus norvegicus CoA + N1-acetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermine physiological substrate, lower rate than with spermidine Rattus norvegicus CoA + N1-acetylspermine
-
 ir
2.3.1.57 acetyl-CoA + spermine spermidine and spermine may be physiological substrates, enzyme may play an important role in interconversion of polyamines Rattus norvegicus CoA + N1-acetylspermine
-
 ?
2.3.1.57 acetyl-CoA + sym-norspermidine high affinity for sym-norspermidine Rattus norvegicus CoA + N1-acetyl-sym-norspermidine
-
 ?
2.3.1.57 additional information not: histone Rattus norvegicus ?
-
 ?
2.3.1.57 additional information not: putrescine Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.57 dimer 2 * 60000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.57 30
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.57 45
-
 half-life: 2.2 min, in 50 mM Tris-HCl, pH 7.5, 10 mM spermidine Rattus norvegicus
2.3.1.57 52
-
 half-life: 0.5 min, in 50 mM Tris-HCl, pH 7.5, 10 mM spermidine Rattus norvegicus
2.3.1.57 60
-
 0.5 min, 90% loss of activity, in 50 mM Tris-HCl, pH 7.5, 10 mM spermidine Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.57 0.04
-
 CoA
-
 Rattus norvegicus