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Literature summary extracted from

  • Morand, L.Z.; Patil, S.; Quasney, M.; German, J.B.
    Alteration of the fatty acid substrate specificity of lysophosphatidate acyltransferase by site-directed mutagenesis (1998), Biochem. Biophys. Res. Commun., 244, 79-84.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.51 isolation of the JC201-plsC gene fragment containing the entire sequence of the enzyme. Complementation of the JC201 phenotype: transformation of JC201 cells with pplSC6 or pplsC6.3. Transformation of XL1-Blue cells with the control plasmid pplsC6.3, and the experimental plasmids pplsC6.3A or pplsC6.3L Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.51 additional information adenine-440 is responsible for the temperature sensitive lesion in the enzyme activity of JC201. Conversion of the mutant adenine-440 back to the native guanine-440 nucleotide restores the enzyme activity at the non-permissive temperature of 42°C Escherichia coli
2.3.1.51 T122A the change increases the substrate specificity in vitro for oleoyl-CoA and linoleoyl-CoA Escherichia coli
2.3.1.51 T122L the change increases the substrate specificity in vitro for lignoceroyl-CoA Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.51 Escherichia coli
-
 XL1-Blue cells
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.51 acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 behenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-behenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 lignoceroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-lignoceroyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 linolenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-linolenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.51 35
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.51 9
-
 assay at Escherichia coli