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Literature summary extracted from

  • Weier, D.; Luhs, W.; Dettendorfer, J.; Frentzen, M.
    sn-1-Acylglycerol-3-phosphate acyltransferase of Escherichia coli causes insertion of cis-11 eicosenoic acid into the sn-2 position of transgenic rapeseed oil (1998), Mol. Breed., 4, 39-46.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.51 modification of the gene of Escherichia coli which encodes the enzyme by inserting an endoplasmic reticulum retrieval signal to its 3'end and introduccion into rapeseed plants under the control of a napin promotor Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.51 Brassica napus
-
-
-
2.3.1.51 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.51 capryl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-capryl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 capryl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Brassica napus CoA + 1-acyl-2-capryl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 cis-11-eicosenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-cis-11 eicosenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 cis-11-eicosenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Brassica napus CoA + 1-acyl-2-cis-11 eicosenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 erucoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-erucoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 erucoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Brassica napus CoA + 1-acyl-2-erucoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Brassica napus CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 additional information an introduced chimeric enzyme gene of E. coli alters the stereochemical composition of transgenic HEAR oil by preferentially directing cis-11 eicosenoic acid to its sn-2 position Escherichia coli ?
-
 ?
2.3.1.51 oleoyl-ACP + sn-1-oleoylglycerol 3-phosphate microsomal fractions from developing, transgenic seeds but not of untransformed plants Escherichia coli ACP + sn-1,2-dioleoylglycerol 3-phosphate
-
 ?
2.3.1.51 oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Brassica napus CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Brassica napus CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.51 30
-
 assay at Escherichia coli
2.3.1.51 30
-
 assay at Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.51 9
-
 assay at Escherichia coli
2.3.1.51 9
-
 assay at Brassica napus