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Literature summary extracted from

  • Ikushiro, H.; Hayashi, H.; Kagamiyama, H.
    Bacterial serine palmitoyltransferase: a water-soluble homodimeric prototype of the eukaryotic enzyme (2003), Biochim. Biophys. Acta, 1647, 116-120.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.50 analysis useful as a model to elucidate the reaction mechanism Sphingomonas paucimobilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.50 overexpression in Escherichia coli BL21 (DE3) pLysS, DNA sequence determination Sphingomonas paucimobilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.50 cytosol
-
 Sphingomonas paucimobilis 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.50 45041
-
 2 * 45041, DNA sequence determination Sphingomonas paucimobilis
2.3.1.50 50000
-
 2 * 50000, SDS-PAGE Sphingomonas paucimobilis
2.3.1.50 90000
-
 gel filtration Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine Sphingomonas paucimobilis rate-limiting enzyme in synthesis of sphingolipids CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.50 Sphingomonas paucimobilis Q93UV0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.50 native and recombinant from E. coli, LCB1 and LCB2 co-immunoprecipitate Sphingomonas paucimobilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.50 palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 formation of the external aldimine intermediate Sphingomonas paucimobilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine
-
 Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine rate-limiting enzyme in synthesis of sphingolipids Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.50 dimer 2 * 50000, SDS-PAGE Sphingomonas paucimobilis
2.3.1.50 dimer 2 * 45041, DNA sequence determination Sphingomonas paucimobilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.50 pyridoxal 5'-phosphate
-
 Sphingomonas paucimobilis