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Literature summary extracted from

  • Ikushiro, H.; Hayashi, H.; Kagamiyama, H.
    A water-soluble homodimeric serine palmitoyltransferase from Sphingomonas paucimobilis EY2395T strain: purification, characterization, cloning, and overproduction (2001), J. Biol. Chem., 276, 18249-18256.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.50 analysis useful as a model to elucidate the reaction mechanism Sphingomonas paucimobilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.50 overexpression in Escherichia coli BL21 (DE3) pLysS, DNA sequence determination Sphingomonas paucimobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.50 3-hydroxypropionate competitive to L-serine Sphingomonas paucimobilis
2.3.1.50 alpha-methyl-DL-serine competitive to L-serine Sphingomonas paucimobilis
2.3.1.50 cysteine competitive to L-serine Sphingomonas paucimobilis
2.3.1.50 additional information not inhibited by halide ions Sphingomonas paucimobilis
2.3.1.50 O-phosphoserine competitive to L-serine Sphingomonas paucimobilis
2.3.1.50 palmitoyl-CoA no inhibition at concentrations up to 10 mM Sphingomonas paucimobilis
2.3.1.50 serine methylester competitive to L-serine Sphingomonas paucimobilis
2.3.1.50 threonine competitive to L-serine Sphingomonas paucimobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.50 0.87
-
 palmitoyl-CoA native and recombinant enzyme Sphingomonas paucimobilis
2.3.1.50 4.2
-
 L-serine native enzyme Sphingomonas paucimobilis
2.3.1.50 10.6
-
 L-serine recombinant enzyme Sphingomonas paucimobilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.50 cytosol
-
 Sphingomonas paucimobilis 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.50 additional information
-
 amino acid sequence determination Sphingomonas paucimobilis
2.3.1.50 44916
-
 2 * 44916, electrospray mass spectrometry Sphingomonas paucimobilis
2.3.1.50 50000
-
 2 * 50000, SDS-PAGE Sphingomonas paucimobilis
2.3.1.50 90000
-
 gel filtration Sphingomonas paucimobilis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.50 Sphingomonas paucimobilis
-
-
-
2.3.1.50 Sphingomonas paucimobilis EY2395
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.50
-
 Sphingomonas paucimobilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.50 palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 ordered bi-bi mechanism Sphingomonas paucimobilis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.50 0.7
-
 purified enzyme Sphingomonas paucimobilis

Storage Stability

EC Number Storage Stability Organism
2.3.1.50 4°C, Tris-HCl 20 mM, in sterile capped vials, 2 months without loss of activity Sphingomonas paucimobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.50 arachidoyl-CoA + L-serine 37% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxydocosan-3-one + CO2
-
 ?
2.3.1.50 elaidoyl-CoA + L-serine 39% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxy-trans-11-eicosen-3-one + CO2
-
 ?
2.3.1.50 lauroyl-CoA + L-serine 18% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxytetradecan-3-one + CO2
-
 ?
2.3.1.50 myristoleoyl-CoA + L-serine 46% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxy-cis-11-hexadecen-3-one + CO2
-
 ?
2.3.1.50 myristoyl-CoA + L-serine 75% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxyhexadecan-3-one + CO2
-
 ?
2.3.1.50 myristoyl-CoA + L-serine 75% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis EY2395 CoA + 2-amino-1-hydroxyhexadecan-3-one + CO2
-
 ?
2.3.1.50 n-heptadecanoyl-CoA + L-serine 75% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxynonadecan-3-one + CO2
-
 ?
2.3.1.50 n-heptadecanoyl-CoA + L-serine 75% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis EY2395 CoA + 2-amino-1-hydroxynonadecan-3-one + CO2
-
 ?
2.3.1.50 oleoyl-CoA + L-serine 57% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxy-11-cis-eicosen-3-one + CO2
-
 ?
2.3.1.50 palmitoleoyl-CoA + L-serine 80% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxy-cis-11-octadecen-3-one + CO2
-
 ?
2.3.1.50 palmitoleoyl-CoA + L-serine 80% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis EY2395 CoA + 2-amino-1-hydroxy-cis-11-octadecen-3-one + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine palmitoyl-CoA is the preferred substrate Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine no other amino acids can substitute for serine Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine palmitoyl-CoA is the preferred substrate Sphingomonas paucimobilis EY2395 CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine no other amino acids can substitute for serine Sphingomonas paucimobilis EY2395 CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
2.3.1.50 stearoyl-CoA + L-serine 51% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxyeicosan-3-one + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.50 dimer 2 * 50000, SDS-PAGE Sphingomonas paucimobilis
2.3.1.50 dimer 2 * 44916, electrospray mass spectrometry Sphingomonas paucimobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.50 37
-
 assay at Sphingomonas paucimobilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.50 0.0388
-
 palmitoyl-CoA
-
 Sphingomonas paucimobilis
2.3.1.50 0.0503
-
 palmitoyl-CoA recombinant enzyme Sphingomonas paucimobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.50 7.5 8
-
 Sphingomonas paucimobilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.50 pyridoxal 5'-phosphate bound to epsilon-amino group of a lysine residue in the active site Sphingomonas paucimobilis