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Literature summary extracted from

  • Gable, K.; Slife, H.; Bacikova, D.; Monaghan, E.; Dunn, T.M.
    Tsc3p is an 80-amino acid protein associated with serine palmitoyltransferase and required for optimal enzyme activity (2000), J. Biol. Chem., 275, 7597-7603.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.50 gene tsc3 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.50 additional information tsc3 mutants show reduced enzyme activity, tsc3 is not required for expression, stability and membrane association of Lcb1p and Lcbp2 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.50 microsome membrane associated Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine Saccharomyces cerevisiae
-
 CoA + 3-dehydro-D-sphinganine + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.50 Saccharomyces cerevisiae
-
 genes LCB1/TSC2, LCB2/TSC1, TSC3
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.50 additional information
-
 activity in wild-type and tsc3 mutants Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine
-
 Saccharomyces cerevisiae CoA + 3-dehydro-D-sphinganine + CO2
-
 ir

Subunits

EC Number Subunits Comment Organism
2.3.1.50 More Lcbp1 and Lcp2 are 2 subunits of the enzyme, tightly associated and not stable independently, tsc3 protein is loosely associated to them Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.50 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.50 8.3
-
 assay at Saccharomyces cerevisiae