Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lynch, D.V.; Fairfield, S.R.
    Sphingolipid long-chain base synthesis in plants. Characterization of serine palmitoyltransferase activity in squash fruit microsomes (1993), Plant Physiol., 103, 1421-1429.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.50 dithiothreitol required for enzyme activity Cucurbita pepo
2.3.1.50 EDTA required for enzyme activity Cucurbita pepo

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.50 beta-chloro-L-alanine mechanism-based inhibition Cucurbita pepo
2.3.1.50 L-Cycloserine mechanism-based inhibition Cucurbita pepo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.50 1.8
-
 L-serine
-
 Cucurbita pepo

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.50 endoplasmic reticulum
-
 Cucurbita pepo 5783
-
2.3.1.50 microsome
-
 Cucurbita pepo
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine Cucurbita pepo
-
 CoA + 3-dehydro-D-sphinganine + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.50 Cucurbita pepo
-
 cv. early profilic straightneck
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.50 fruit age-dependent amount of enzyme activity Cucurbita pepo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.50 0.057 0.084
-
 Cucurbita pepo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine
-
 Cucurbita pepo CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine palmitoyl-CoA is the preferred substrate Cucurbita pepo CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine optimal palmitoyl-CoA concentration is 0.2 mM Cucurbita pepo CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine palmitoyl-CoA is used in preference to other saturated or unsaturated acyl-CoA substrates Cucurbita pepo CoA + 3-dehydro-D-sphinganine + CO2
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.50 36 40
-
 Cucurbita pepo

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.50 7.6
-
-
 Cucurbita pepo

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.50 pyridoxal 5'-phosphate stimulates Cucurbita pepo