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Literature summary extracted from

  • Williams, R.D.; Wang, E.; Merrill, A.H.
    Enzymology of long-chain base synthesis by liver: characterization of serine palmitoyltransferase in rat liver microsomes (1984), Arch. Biochem. Biophys., 228, 282-291.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.50 dithiothreitol required for enzyme activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.50 palmitoyl-CoA 0.5-1.0 mM, substrate inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.50 0.67
-
 L-serine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.50 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.50 additional information NaCl, LiCl, MgCl2, MnCl2 have no effect on activity Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine Rattus norvegicus first step of biosynthesis of sphingolipid bases CoA + 3-dehydro-D-sphinganine + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.50 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.50 additional information carrier lipid during extraction required for activity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.50 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.50 0.000063
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.50 additional information specificity Rattus norvegicus ?
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine palmitoyl-CoA is the preferred substrate Rattus norvegicus CoA + 3-dehydro-D-sphinganine + CO2
-
 ir
2.3.1.50 palmitoyl-CoA + L-serine first step of biosynthesis of sphingolipid bases Rattus norvegicus CoA + 3-dehydro-D-sphinganine + CO2
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.50 50
-
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.50 37
-
 30 min, stable Rattus norvegicus
2.3.1.50 50
-
 above, precipitation of enzyme Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.50 8
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.50 6.7 8.6 about 50% of activity maximum at pH 6.7 and pH 8.6 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.50 pyridoxal 5'-phosphate stimulates Rattus norvegicus