Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Barenholz, Y.; Edelman, I.; Gatt, S.
    Amine:acetyl CoenzymeA acetyltransferase from the soluble fraction of Hansenula ciferri: isolation and properties (1974), Biochim. Biophys. Acta, 358, 262-274.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.5 albumin activation, with long-chain primary amines as substrate Wickerhamomyces ciferrii

General Stability

EC Number General Stability Organism
2.3.1.5 glycerol, 10% v/v, stabilizes Wickerhamomyces ciferrii
2.3.1.5 sucrose or mannitol, 0.1 M, cannot fully replace glycerol Wickerhamomyces ciferrii
2.3.1.5 Tris-buffer cannot replace phosphate buffer during purification Wickerhamomyces ciferrii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.5 AgNO3
-
 Wickerhamomyces ciferrii
2.3.1.5 cholate
-
 Wickerhamomyces ciferrii
2.3.1.5 coenzyme A
-
 Wickerhamomyces ciferrii
2.3.1.5 deoxycholate
-
 Wickerhamomyces ciferrii
2.3.1.5 dithiothreitol above 0.5 mM, not Wickerhamomyces ciferrii
2.3.1.5 DTNB
-
 Wickerhamomyces ciferrii
2.3.1.5 EDTA partly reversible by MgCl2 Wickerhamomyces ciferrii
2.3.1.5 additional information no inhibition by acetylated products and 2-mercaptoethanol Wickerhamomyces ciferrii
2.3.1.5 p-hydroxymercuribenzoate
-
 Wickerhamomyces ciferrii
2.3.1.5 p-mercuribenzoate
-
 Wickerhamomyces ciferrii
2.3.1.5 palmitoyl-CoA
-
 Wickerhamomyces ciferrii
2.3.1.5 taurocholate
-
 Wickerhamomyces ciferrii
2.3.1.5 Triton X-100
-
 Wickerhamomyces ciferrii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 additional information
-
 additional information reaction kinetics Wickerhamomyces ciferrii
2.3.1.5 0.07
-
 tetradecylamine
-
 Wickerhamomyces ciferrii
2.3.1.5 0.08
-
 octylamine
-
 Wickerhamomyces ciferrii
2.3.1.5 0.14
-
 Hexylamine
-
 Wickerhamomyces ciferrii
2.3.1.5 0.14 0.145 p-tyramine
-
 Wickerhamomyces ciferrii
2.3.1.5 0.28 0.37 4-nitroaniline
-
 Wickerhamomyces ciferrii
2.3.1.5 0.3
-
 dodecylamine
-
 Wickerhamomyces ciferrii
2.3.1.5 0.42
-
 serotonin
-
 Wickerhamomyces ciferrii
2.3.1.5 0.53
-
 tryptamine pineal gland Wickerhamomyces ciferrii
2.3.1.5 0.71 0.72 hydroxytyramine
-
 Wickerhamomyces ciferrii
2.3.1.5 0.72
-
 histamine
-
 Wickerhamomyces ciferrii
2.3.1.5 0.77 0.8 decylamine
-
 Wickerhamomyces ciferrii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.5 cytosol
-
 Wickerhamomyces ciferrii 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Wickerhamomyces ciferrii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5 partial Wickerhamomyces ciferrii

Storage Stability

EC Number Storage Stability Organism
2.3.1.5 -20°C, at least 2 years in the presence of 10% glycerol v/v Wickerhamomyces ciferrii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + 4-nitroaniline
-
 Wickerhamomyces ciferrii CoA + 4-nitroacetanilide
-
 ?
2.3.1.5 acetyl-CoA + D-glucosamine
-
 Wickerhamomyces ciferrii CoA + N-acetyl-D-glucosamine
-
 ?
2.3.1.5 acetyl-CoA + decylamine
-
 Wickerhamomyces ciferrii CoA + N-acetyldecylamine
-
 ?
2.3.1.5 acetyl-CoA + dodecylamine
-
 Wickerhamomyces ciferrii CoA + N-acetyldodecylamine
-
 ?
2.3.1.5 acetyl-CoA + hexylamine
-
 Wickerhamomyces ciferrii CoA + N-acetylhexylamine
-
 ?
2.3.1.5 acetyl-CoA + histamine
-
 Wickerhamomyces ciferrii CoA + N-acetylhistamine
-
 ?
2.3.1.5 acetyl-CoA + hydroxytyramine
-
 Wickerhamomyces ciferrii CoA + ?
-
 ?
2.3.1.5 acetyl-CoA + long-chain primary amines C6-C16 Wickerhamomyces ciferrii CoA + ?
-
 ?
2.3.1.5 acetyl-CoA + noradrenaline
-
 Wickerhamomyces ciferrii CoA + ?
-
 ?
2.3.1.5 acetyl-CoA + octylamine
-
 Wickerhamomyces ciferrii CoA + N-acetyloctylamine
-
 ?
2.3.1.5 acetyl-CoA + p-tyramine
-
 Wickerhamomyces ciferrii CoA + N-acetyl-p-tyramine
-
 ?
2.3.1.5 acetyl-CoA + serotonin
-
 Wickerhamomyces ciferrii CoA + N-acetylserotonin
-
 ?
2.3.1.5 acetyl-CoA + tetradecylamine
-
 Wickerhamomyces ciferrii CoA + N-acetyltetradecylamine
-
 ?
2.3.1.5 acetyl-CoA + tryptamine
-
 Wickerhamomyces ciferrii CoA + N-acetyltryptamine
-
 ?
2.3.1.5 additional information no substrate: polyamines, amino acids, sphingosine bases, ethanolamine, C1-C4 primary amines, some secondary and tertiary amines Wickerhamomyces ciferrii ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 additional information
-
 pH-optimum depends on buffer Wickerhamomyces ciferrii
2.3.1.5 7.3
-
 tricine buffer Wickerhamomyces ciferrii
2.3.1.5 7.5
-
 potassium phosphate or piperazine-N,N-bis(2-ethanolsulfonic acid) buffer Wickerhamomyces ciferrii
2.3.1.5 8.3
-
 N-tris(hydroxymethyl)methyl-2-aminoethane buffer Wickerhamomyces ciferrii
2.3.1.5 9.5
-
 cyclohexylamineopropanesulfonic acid buffer Wickerhamomyces ciferrii
2.3.1.5 9.8
-
 trisethanolamine buffer Wickerhamomyces ciferrii