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Literature summary extracted from

  • Izumi, Y.; Morita, H.; Tani, Y.; Ogata, K.
    Partial purification and some properties of a 7-keto-8-aminopelargonic acid synthetase, an enzyme involved in biotin biosynthesis (1973), Agric. Biol. Chem., 37, 1327-1333.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.47 1,10-phenanthroline to some extent Lysinibacillus sphaericus
2.3.1.47 Ag+
-
 Lysinibacillus sphaericus
2.3.1.47 Cd2+ weak Lysinibacillus sphaericus
2.3.1.47 citrate to some extent Lysinibacillus sphaericus
2.3.1.47 Co2+
-
 Lysinibacillus sphaericus
2.3.1.47 Cu2+
-
 Lysinibacillus sphaericus
2.3.1.47 D-alanine
-
 Lysinibacillus sphaericus
2.3.1.47 D-cycloserine
-
 Lysinibacillus sphaericus
2.3.1.47 D-penicillamine L-enantiomer not as effective Lysinibacillus sphaericus
2.3.1.47 D/L-histidine
-
 Lysinibacillus sphaericus
2.3.1.47 diisopropyl fluorophosphate to some extent Lysinibacillus sphaericus
2.3.1.47 glycine
-
 Lysinibacillus sphaericus
2.3.1.47 hydroxylamine
-
 Lysinibacillus sphaericus
2.3.1.47 isoniazide to some extent Lysinibacillus sphaericus
2.3.1.47 L-cysteine strong Lysinibacillus sphaericus
2.3.1.47 L-serine
-
 Lysinibacillus sphaericus
2.3.1.47 additional information no inhibition by HgCl2, EDTA, 2,2'-dipyridyl, oxalate, KCN, various amino acids Lysinibacillus sphaericus
2.3.1.47 Ni2+
-
 Lysinibacillus sphaericus
2.3.1.47 phenylhydrazine
-
 Lysinibacillus sphaericus
2.3.1.47 Semicarbazide
-
 Lysinibacillus sphaericus
2.3.1.47 Zn2+ weak Lysinibacillus sphaericus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.47 additional information no metal ion requirement Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.47 Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.47 partial Lysinibacillus sphaericus
2.3.1.47 recombinant from E. coli overproducing strain Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.47 0.00225
-
 partially purified enzyme Lysinibacillus sphaericus

Storage Stability

EC Number Storage Stability Organism
2.3.1.47 -20°C, 0.01 M phosphate buffer, pH 7.0, 5 mM 2-mercaptoethanol, 5 months Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine absolutely specific for L-alanine Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.47 KAPA synthetase
-
 Lysinibacillus sphaericus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.47 37
-
 assay at Lysinibacillus sphaericus
2.3.1.47 60
-
-
 Lysinibacillus sphaericus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.47 45 65 about half-maximal activity at 45°C and 65°C Lysinibacillus sphaericus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.47 60
-
 and below, stable Lysinibacillus sphaericus
2.3.1.47 70
-
 t1/2: 10 min Lysinibacillus sphaericus
2.3.1.47 80
-
 and above, inactivation Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.47 7
-
-
 Lysinibacillus sphaericus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.47 6.5 7.5 about half-maximal activity at pH 6.5 and pH 7.5 Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.47 pyridoxal 5'-phosphate requirement, pyridoxal-5'-phosphate protein, not replaceable by pyridoxal, pyridoxine, pyridoxine 5-phosphate, pyridoxamine, pyridoxamine 5-phosphate Lysinibacillus sphaericus