Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Born, T.L.; Blanchard, J.S.
    Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase (1999), Biochemistry, 38, 14416-14423.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.46 overexpression of metA gene in Escherichia coli BL21 (DE3) Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.46 iodoacetamide inhibition is pH-dependent Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.46 0.17
-
succinyl-CoA recombinant protein Escherichia coli
2.3.1.46 0.18
-
glutaryl-CoA recombinant protein Escherichia coli
2.3.1.46 0.64
-
coenzyme A recombinant protein Escherichia coli
2.3.1.46 1.6
-
L-homoserine recombinant protein Escherichia coli
2.3.1.46 3.5
-
O-succinylhomoserine recombinant protein Escherichia coli
2.3.1.46 10
-
D-homoserine recombinant protein Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.46 35600
-
2 * 35600, SDS-PAGE Escherichia coli
2.3.1.46 86000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.46 succinyl-CoA + L-homoserine Escherichia coli involved in cystathionine and methionine biosynthesis CoA + O-succinyl-L-homoserine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.46 Escherichia coli
-
metA gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.46 recombinant from E. coli BL21 (DE3) Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.46 succinyl-CoA + L-homoserine = CoA + O-succinyl-L-homoserine ping-pong mechanism, enzyme contains a cysteine in the active site, member of the acyltransferase family Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.46 glutaryl-CoA + L-homoserine
-
Escherichia coli CoA + O-glutaryl-L-homoserine
-
r
2.3.1.46 additional information 3-amino-1-propanol, L-serine, and L-threonine are poor substrates Escherichia coli ?
-
?
2.3.1.46 additional information no activity with acetyl-CoA, propionyl-CoA, butyryl-CoA, crotonyl-CoA, malonyl-CoA, and gamma-hydroxybutyric acid, and gamma-aminobutyric acid Escherichia coli ?
-
?
2.3.1.46 succinyl-CoA + D-homoserine
-
Escherichia coli CoA + O-succinyl-D-homoserine
-
r
2.3.1.46 succinyl-CoA + L-homoserine
-
Escherichia coli CoA + O-succinyl-L-homoserine
-
r
2.3.1.46 succinyl-CoA + L-homoserine involved in cystathionine and methionine biosynthesis Escherichia coli CoA + O-succinyl-L-homoserine
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.46 dimer 2 * 35600, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.46 1.6
-
glutaryl-CoA recombinant protein Escherichia coli
2.3.1.46 5.23
-
coenzyme A recombinant protein Escherichia coli
2.3.1.46 5.23
-
O-succinyl-L-homoserine recombinant protein Escherichia coli
2.3.1.46 12
-
D-homoserine recombinant protein Escherichia coli
2.3.1.46 24
-
succinyl-CoA recombinant protein Escherichia coli
2.3.1.46 24
-
L-homoserine recombinant protein Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.46 additional information
-
broad maximum Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.46 additional information
-
pKs of 6.6 and about 7.9 Escherichia coli
2.3.1.46 6 8.7
-
Escherichia coli