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Literature summary extracted from

  • Jin, L.; Lee, Y.P.; Jonas, A.
    Biochemical and biophysical characterization of human recombinant lecithin: cholesterol acyltransferase (1997), J. Lipid Res., 38, 1085-1093.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.43 expression in Chinese hamster ovary cells, recombinant enzyme is structurally similar to plasma LCAT Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.43 additional information
-
 mass spectrometry analysis gives a range from 55700 to 59600 Homo sapiens
2.3.1.43 49000
-
 x * 49000, denatured and deglycosylated enzyme Homo sapiens
2.3.1.43 73000
-
 x * 73000, recombinant enzyme, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.43 glycoprotein glycosylation in a recombinant enzyme is more extensive than that of isolated from plasma Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.43 purification of recombinant enzymes Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.43 additional information
-
 recombinant enzyme is half as enzymatically active as plasma LCAT Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 phosphatidylcholine + sterol
-
 Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.43 ? x * 73000, recombinant enzyme, SDS-PAGE Homo sapiens
2.3.1.43 ? x * 49000, denatured and deglycosylated enzyme Homo sapiens