Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Collet, X.; Fielding, C.J.
    Effects of inhibitors of N-linked oligosaccharide processing on the secretion, stability, and activity of lecithin:cholesterol acyltransferase (1991), Biochemistry, 30, 3228-3234.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.43 castanospermine reduces the maximum reaction velocity but not the Km Homo sapiens
2.3.1.43 deoxynojirimycin reduces the maximum reaction velocity but not the Km Homo sapiens
2.3.1.43 methyldeoxynojirimycin reduces the maximum reaction velocity but not the Km Homo sapiens
2.3.1.43 tunicamycin reduces the maximum reaction velocity but not the Km Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.43 21.4
-
 cholesterol in cholesterol-lecithin vesicles Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.43 46000
-
 x * 46000, SDS-PAGE after cleavage of the N-linked carbohydrates Homo sapiens
2.3.1.43 67000
-
 x * 67000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.43 glycoprotein almost all of the carbohydrate moiety of LCAT is N-linked with part of the high-mannose and part of the complex type Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.43 precipitation from plasma and purification by phenyl-agarose, DEAE-cellulose and hydroxyapatite columns Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.43 plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 phosphatidylcholine + sterol
-
 Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.43 ? x * 67000, SDS-PAGE Homo sapiens
2.3.1.43 ? x * 46000, SDS-PAGE after cleavage of the N-linked carbohydrates Homo sapiens