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Literature summary extracted from

  • Albers, J.J.; Chen, C.H.; Lacko, A.G.
    Isolation, characterization, and assay of lecithin-cholesterol acyltransferase (1986), Methods Enzymol., 129, 763-783.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.43 apolipoprotein A-I absolute requirement with phosphatidylcholine-cholesterol vesicles as a substrate Homo sapiens
2.3.1.43 additional information apo-A-II, apo-C-III and apo-D inhibit activation of enzyme by apo-A-1 Homo sapiens

General Stability

EC Number General Stability Organism
2.3.1.43 inactivation at the air-water interface is prevented by buffered media of low ionic strength Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.43 5,5'-dithiobis(2-nitrobenzoic acid)
-
Homo sapiens
2.3.1.43 carnitine
-
Homo sapiens
2.3.1.43 chlorpromazine
-
Homo sapiens
2.3.1.43 cysteine
-
Homo sapiens
2.3.1.43 diisopropyl fluorophosphate
-
Homo sapiens
2.3.1.43 p-hydroxymercuribenzoate
-
Homo sapiens
2.3.1.43 reduced glutathione
-
Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.43 65000
-
x * 65000, SDS-PAGE Homo sapiens
2.3.1.43 67000
-
size exclusion chromatography Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.43 phosphatidylcholine + sterol Homo sapiens enzyme is believed to act on the surface of high-density lipoproteins, catalyzes the hydrolysis of fatty acids of the sn-2-position of phosphatidylcholine and transfers the fatty acid to free cholesterol to form cholesteryl ester, transesterification is the major source of plasma cholesteryl ester in human, enzyme has the key role in the transport of cholesterol from peripheral tissues to the liver, in the interconversion of HDL subclasses and the maintenance of lipoprotein structure 1-acylglycerophosphocholine + sterol ester
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.43 glycoprotein 25% carbohydrate Homo sapiens
2.3.1.43 glycoprotein with a 5% sialic acid content Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.43 plasma
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.43 0.54
-
at 37°C and pH: 7.4 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.3.1.43 4°C, 0.4 mM phosphate buffer, 4 mM 2-mercaptoethanol, under N2, 4 weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 additional information lecithin-cholesterol single bilayer vesicles as substrate Homo sapiens ?
-
?
2.3.1.43 phosphatidylcholine + sterol cholesterol as substrate Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
r
2.3.1.43 phosphatidylcholine + sterol enzyme is believed to act on the surface of high-density lipoproteins, catalyzes the hydrolysis of fatty acids of the sn-2-position of phosphatidylcholine and transfers the fatty acid to free cholesterol to form cholesteryl ester, transesterification is the major source of plasma cholesteryl ester in human, enzyme has the key role in the transport of cholesterol from peripheral tissues to the liver, in the interconversion of HDL subclasses and the maintenance of lipoprotein structure Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.43 ? x * 65000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.43 8
-
transesterification of high-density lipoproteins and liposomes Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.43 apolipoprotein A1
-
Homo sapiens