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Literature summary extracted from

  • Webber, K.O.; Hajra, A.K.
    Purification of dihydroxyacetone phosphate acyltransferase from guinea pig liver peroxisomes (1993), Arch. Biochem. Biophys., 300, 88-97.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.42 Phospholipid activation, e.g. phosphatidylcholine, dilauroylphosphatidylcholine Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.42 CoA
-
 Cavia porcellus
2.3.1.42 coenzyme A kinetics Cavia porcellus
2.3.1.42 N-ethylmaleimide weak Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.42 0.061 0.078 dihydroxyacetone phosphate
-
 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.42 peroxisome
-
 Cavia porcellus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.42 69000
-
 x * 69000, SDS-PAGE Cavia porcellus
2.3.1.42 90000
-
 gel filtration Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.42 palmitoyl-CoA + dihydroxyacetone phosphate Cavia porcellus initiates glycerolipid, especially ether-linked glycerolipid, biosynthesis in higher eukaryotes CoA + monopalmitoyldihydroxyacetone phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.42 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.42 with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, i.e. CHAPS, plus 0.15 M NaCl Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.42 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.42 2.47
-
 purified enzyme Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.42 acyl-CoA + dihydroxyacetone phosphate
-
 Cavia porcellus CoA + acyldihydroxyacetone phosphate i.e. acylglycerone phosphate ?
2.3.1.42 palmitoyl-CoA + dihydroxyacetone phosphate
-
 Cavia porcellus CoA + monopalmitoyldihydroxyacetone phosphate
-
 ?
2.3.1.42 palmitoyl-CoA + dihydroxyacetone phosphate initiates glycerolipid, especially ether-linked glycerolipid, biosynthesis in higher eukaryotes Cavia porcellus CoA + monopalmitoyldihydroxyacetone phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.42 ? x * 69000, SDS-PAGE Cavia porcellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.42 37
-
 assay at Cavia porcellus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.42 0.76
-
 CoA
-
 Cavia porcellus