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Literature summary extracted from

  • Jones, C.L.; Hajra, A.K.
    Properties of guinea pig liver peroxisomal dihydroxyacetone phosphate acyltransferase (1980), J. Biol. Chem., 255, 8289-8295.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.42 asolectin activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol Cavia porcellus
2.3.1.42 bovine serum albumin activation, by inhibiting the formation of palmitoyl-CoA micelles Cavia porcellus
2.3.1.42 cholate
-
 Cavia porcellus
2.3.1.42 Detergents stimulation and solubilization of membrane-bound microsomal activity, e.g. Lubrol WX, cholate and deoxycholate Cavia porcellus
2.3.1.42 F- activation, membrane-bound enzyme Cavia porcellus
2.3.1.42 Triton X-100
-
 Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.42 Cu2+
-
 Cavia porcellus
2.3.1.42 glycerol 3-phosphate pH 6.0-7.0 Cavia porcellus
2.3.1.42 N-ethylmaleimide not peroxisomal enzyme Cavia porcellus
2.3.1.42 palmitoyl-CoA above 0.08 mM; bovine serum albumin protects Cavia porcellus
2.3.1.42 Tris-maleate buffer pH 5.0-6.0 Cavia porcellus
2.3.1.42 Trypsin
-
 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.42 additional information
-
 additional information
-
 Cavia porcellus
2.3.1.42 0.04
-
 palmitoyl-CoA pH 5.5 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.42 mitochondrion
-
 Cavia porcellus 5739
-
2.3.1.42 additional information subcellular distribution Cavia porcellus
-
-
2.3.1.42 additional information not: cytosol Cavia porcellus
-
-
2.3.1.42 peroxisome
-
 Cavia porcellus 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.42 additional information no activation by Mg2+, partially purified Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.42 Cavia porcellus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.42 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.42 additional information
-
-
 Cavia porcellus

Storage Stability

EC Number Storage Stability Organism
2.3.1.42 -20°C, up to 1 month Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.42 acyl-CoA + dihydroxyacetone phosphate
-
 Cavia porcellus CoA + acyldihydroxyacetone phosphate i.e. acylglycerone phosphate ?
2.3.1.42 palmitoyl-CoA + dihydroxyacetone phosphate
-
 Cavia porcellus CoA + monopalmitoyldihydroxyacetone phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.42 37
-
 assay at Cavia porcellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.42 additional information
-
 membrane-bound: heat-stable Cavia porcellus
2.3.1.42 additional information
-
 solubilized: heat-labile Cavia porcellus
2.3.1.42 48
-
 t1/2: 13 min Cavia porcellus
2.3.1.42 55
-
 15 min stable, crude peroxisomal preparation Cavia porcellus
2.3.1.42 60
-
 10 min stable Cavia porcellus
2.3.1.42 60
-
 crude peroxisomal preparation Cavia porcellus
2.3.1.42 65
-
 inactivation within 15 min, crude peroxisomal preparation Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.42 5.5
-
 membrane-bound peroxisomal enzyme Cavia porcellus
2.3.1.42 5.5
-
 optimum without detergent, no activity at pH 5.5 in presence of cholate Cavia porcellus
2.3.1.42 7.4
-
 optimum with detergent Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.42 5.5 9 detectable activity in this range Cavia porcellus