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Literature summary extracted from

  • Price, A.C.; Choi, K.H.; Heath, R.J.; Li, Z.; White, S.W.; Rock, C.O.
    Inhibition of beta-ketoacyl-acyl carrier protein synthases by thiolactomycin and cerulenin. Structure and mechanism (2001), J. Biol. Chem., 276, 6551-6559.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.41 synthases I, II, and III, recombinant expression Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.41 structure analysis, three-dimensional model of enzyme complexed with inhibitors thiolactomycin and cerulenin Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 cerulenin analysis of the binding mechanism, mimicks the condensation transition state; FabB: inhibition is irreversible Escherichia coli
2.3.1.41 thiolactomycin analysis of the binding mechanism, mimics malonyl-[acyl-carrier-protein]; FabB: inhibition is reversible, competitive Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Escherichia coli
-
 synthases I, II, and III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 recombinant synthases I, i.e. FabA, II, i.e. FabB, and III, i.e.FabH Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.41 an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] synthaseIII/FabH: His-Asn-Cys catalytic triad Escherichia coli
a
2.3.1.41 an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] synthaseII/FabB: His-HIs-Cys catalytic triad Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?